scalable nucleotide alignment program
,index <input.fa> <output-dir> /[,<options>/]
Welcome to SNAP version 1.0beta.18.
Seed size (default: 20)
Hash table slack (default: 0.3)
Use pre-computed table bias for hg19, which results in better speed, balance, and a smaller index, but only works for the complete human reference.
This parameter is deprecated and will be ignored.
Specify the maximum number of threads to use. Default is the number of cores.
Specify characters to use as chromosome name terminators in the FASTA header line; these characters and anything after are
not part of the chromosome name. You must specify all characters on a single -B switch. So, for example, with -B_|,
the FASTA header line '>chr1|Chromosome 1' would generate a chromosome named 'chr1'. There's a separate flag for
indicating that a space is a terminator.
Indicates that the space character is a terminator for chromosome names (see -B above). This may be used in addition
to other terminators specified by -B. -B and -bSpace are case sensitive.
Specify the number of Ns to put as padding between chromosomes. This must be as large as the largest
edit distance you'll ever use, and there's a performance advantage to have it be bigger than any
read you'll process. Default is 500
Build a histogram of seed popularity. This is just for information, it's not used by SNAP.
Compute hash table sizes exactly. This will slow down index build, but usually will result in smaller indices.
The number of bytes to use for the hash table key. Larger values increase SNAP's memory footprint, but allow larger seeds. Default: 4
Build a larger index that's a little faster, particualrly for runs with quick/inaccurate parameters. Increases index size by
about 30%, depending on the other index parameters and the contents of the reference genome
The size of the genome locations stored in the index. This can be from 4 to 8 bytes. The locations need to be big enough
not only to index the genome, but also to allow some space for representing seeds that occur multiple times. For the
human genome, it will fit with four byte locations if the seed size is 19 or larger, but needs 5 (or more) for smaller
seeds. Making the location size bigger than necessary will just waste (lots of) space, so unless you're doing something
quite unusual, the right answer is 4 or 5. Default is 4
Use a temp file to work better in smaller memory. This only helps a little, but can be the difference if you're close.
In particular, this will generally use less memory than the index will use once it's built, so if this doesn't work you
won't be able to use the index anyway. However, if you've got sufficient memory to begin with, this option will just
slow down the index build by doing extra, useless IO.