SYNOPSIS

mirabait [options] {-b baitfile [-b ...] | -B file | -j joblibrary} {-p file_1 file_2 | -P file3}* [file4 ...]

DESCRIPTION

mirabait selects reads from a read collection which are partly similar or equal to sequences defined as target baits. Similarity is defined by finding a user-adjustable number of common k-mers (sequences of k consecutive bases) which are the same in the bait sequences and the screened sequences to be selected, either in forward or forward/reverse complement direction. Adding a DUST-like repeat filter for repeats up 4 bases is optional.

When used on paired files, selects sequences where at least one mate matches.

OPTIONS

Main options:

-b file

Load bait sequences from file (multiple -b allowed)

-B file

Load baits from kmer statistics file, not from sequence files. Only one -B allowed, cannot be combined with -b. (see -K for creating such a file)

-j job

Set options for predefined job from supplied MIRA library Currently available jobs:

rrna Bait rRNA sequences

-p file1 file2

Load paired sequences to search from file1 and file2 Files must contain same number of sequences, sequence names must be in same order. Multiple -p allowed, but must come before non-paired files.

-P file

Load paired sequences from file File must be interleaved: pairs must follow each other, non-pairs are not allowed. Multiple -p allowed, but must come before non-paired files.

-k int

kmer length of bait in bases (<=256, default=31)

-n int

If >0: minimum number of k-mer baits needed (default=1) If <=0: allowed number of missed kmers over sequence

length

-d

Do not use kmers with microrepeats (DUST-like, see also -D)

-D int

Set length of microrepeats in kmers to discard from bait.
- int > 0 microrepeat len in percentage of kmer length. E.g.: -k 17 -D 67 --> 11.39 bases --> 12 bases.
- int < 0 microrepeat len in bases.
- int != 0 implies -d, int=0 turns DUST filter off.

-i

Selects sequences that do not hit bait

-I

Selects sequences that hit and do not hit bait (to different files)

-r

No checking of reverse complement direction

-t

Number of threads to use (default=0 -> up to 4 CPU cores)

Options for output definition:

Normally mirabait writes separate result files (named 'bait_match_*' and 'bait_miss_*') for each input to the current directory. For changing this behaviour and other relating to output, use these options:

-c

No case change of sequence to denote bait hits

-l int

length of a line (FASTA only, default 0=unlimited)

-K file

Save kmer statistics to 'file' (see also -B)

-N name

Change the prefix 'bait' to <name> Has no effect if -o/-O is used and targets are not directories

-o <path>

Save sequences matching bait to path If path is a directory, write separate files into this directory. If not, combine all matching sequences from the input file(s) into a single file specified by the path.

-O <path>

Like -o, but for sequences not matching

Other options:

-T dir

Use 'dir' as directory for temporary files instead of current working directory.

-m integer

Memory to use for computing kmer statistics
0..100 = use percentage of free system memory
>100 = amount of MiB to use (e.g. 16384 for 16 GiB)
Default 75 (75% of free system memory).

Defining files types to load/save:

Normally mirabait recognises the file types according to the file extension (even when packed). In cases you need to force a certain file type because the file extension is non-standard, use the EMBOSS notation to force a type: <filetype>::<name_of_file>. E.g., to tell that "somefile.dat" is FASTQ, use: fastq::somefile.dat Recognised types are: caf, fasta, fastq, gbf, gbk, gbff, maf and phd.

MIRABAIT will write files in the same file type as the corresponding input files. Examples:

mirabait -b b.fasta file.fastq

mirabait -I -j rrna -p file_1.fastq file_2.fastq

mirabait -b b1.fasta -b b2.gbk file.fastq

mirabait -b fasta::baits.dat -p fastq::file_1.dat fastq::file_2.dat

mirabait -b b.fasta -p file_1.fastq file_2.fastq -P file3.fasta file4.caf

mirabait -I -b b.fasta -p file_1.fastq file_2.fastq -P file3.fasta file4.caf

mirabait -k 27 -n 10 -b b.fasta file.fastq

mirabait -b fasta::b.dat fastq::file.dat

mirabait -o /dev/shm/ -b b.fasta -p file_1.fastq file_2.fastq

mirabait -o ,/dev/shm/match/ -b b.fasta -p file_1.fastq file_2.fastq

mirabait -b human_genome.fasta -K HG_kmerstats.mhs.gz -p file1.fastq file2.fastq

mirabait -B HG_kmerstats.mhs.gz -p file1.fastq file2.fastq

mirabait -d -B HG_kmerstats.mhs.gz -p file1.fastq file2.fastq

AUTHOR

Bastien Chevreux <[email protected]>

This manual page was written by Bastien Chevreux <[email protected]> but can be freely used for any documentation purpose.