VERSION
version 1.70SYNOPSIS
use Bio::ASN1::Sequence::Indexer;
# creating & using the index is just a few lines
my $inx = Bio::ASN1::Sequence::Indexer->new(
-filename => 'seq.idx',
-write_flag => 'WRITE'); # needed for make_index call, but if opening
# existing index file, don't set write flag!
$inx->make_index('seq1.asn', 'seq2.asn');
my $seq = $inx->fetch('AF093062'); # Bio::Seq obj for Sequence (doesn't work yet)
# alternatively, if one prefers just a data structure instead of objects
$seq = $inx->fetch_hash('AF093062'); # a hash produced by Bio::ASN1::Sequence
# that contains all data in the Sequence record
DESCRIPTION
Bio::ASN1::Sequence::Indexer is a Perl Indexer for NCBI Sequence genome databases. It processes an ASN.1-formatted Sequence record and stores the file position for each record in a way compliant with Bioperl standard (in fact its a subclass of Bioperl's index objects).Note that this module does not parse record, because it needs to run fast and grab only the gene ids. For parsing record, use Bio::ASN1::Sequence.
As with Bio::ASN1::Sequence, this module is best thought of as beta version - it works, but is not fully tested.
METHODS
fetch
Parameters: $geneid - id for the Sequence record to be retrieved Example: my $hash = $indexer->fetch(10); # get Sequence #10 Function: fetch the data for the given Sequence id. Returns: A Bio::Seq object produced by Bio::SeqIO::sequence Notes: Bio::SeqIO::sequence does not exist and probably won't exist for a while! So call fetch_hash instead
fetch_hash
Parameters: $seqid - id for the Sequence record to be retrieved Example: my $hash = $indexer->fetch_hash('AF093062'); Function: fetch a hash produced by Bio::ASN1::Sequence for given id Returns: A data structure containing all data items from the Sequence record. Notes: Alternative to fetch()
INTERNAL METHODS
_version
_type_stamp
_index_file
_file_format
_file_handle
Title : _file_handle Usage : $fh = $index->_file_handle( INT ) Function: Returns an open filehandle for the file index INT. On opening a new filehandle it caches it in the @{$index->_filehandle} array. If the requested filehandle is already open, it simply returns it from the array. Example : $fist_file_indexed = $index->_file_handle( 0 ); Returns : ref to a filehandle Args : INT Notes : This function is copied from Bio::Index::Abstract. Once that module changes file handle code like I do below to fit perl 5.005_03, this sub would be removed from this module
PREREQUISITE
Bio::ASN1::Sequence, Bioperl and all dependencies therein.INSTALLATION
Same as Bio::ASN1::EntrezGeneCITATION
Liu, Mingyi, and Andrei Grigoriev. ``Fast parsers for Entrez Gene.'' Bioinformatics 21, no. 14 (2005): 3189-3190.OPERATION SYSTEMS SUPPORTED
Any OS that Perl & Bioperl run on.FEEDBACK
Mailing lists
User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing list. Your participation is much appreciated.
[email protected] - General discussion http://bioperl.org/wiki/Mailing_lists - About the mailing lists
Support
Please direct usage questions or support issues to the mailing list: [email protected]rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible.
Reporting bugs
Report bugs to the Bioperl bug tracking system to help us keep track of the bugs and their resolution. Bug reports can be submitted via the web:
https://redmine.open-bio.org/projects/bioperl/
AUTHOR
Dr. Mingyi Liu <[email protected]>COPYRIGHT
This software is copyright (c) 2005 by Mingyi Liu, 2005 by GPC Biotech AG, and 2005 by Altana Research Institute.This software is available under the same terms as the perl 5 programming language system itself.