SYNOPSIS
use Bio::Assembly::IO;
$in = Bio::Assembly::IO->new(-file=>"<inputfilename",
-format=>'phrap');
$out = Bio::Assembly::IO->new(-file=>">outputfilename",
-format=>'phrap');
while ( my $scaffold = $in->next_assembly() ) {
# do something with Bio::Assembly::Scaffold instance
# ...
$out->write_assembly(-scaffold => $scaffold);
}
$in->close;
$out->close;
DESCRIPTION
Bio::Assembly::IO is a handler module for formats in the Assembly::IO set (e.g. Bio::Assembly::IO::phrap).FEEDBACK
Mailing Lists
User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing lists Your participation is much appreciated.
[email protected] - General discussion http://bioperl.org/wiki/Mailing_lists - About the mailing lists
Support
Please direct usage questions or support issues to the mailing list:rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible.
Reporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via the web:
https://github.com/bioperl/bioperl-live/issues
AUTHOR
Robson Francisco de SouzaE-mail: [email protected]
CONTRIBUTORS
#APPENDIX
The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _new
Title : new Usage : $stream = Bio::Assembly::IO->new( -file => $filename, -format =>'format' ) Function: Returns a new assembly stream Returns : A Bio::Assembly::IO::Handler initialised with the appropriate format Args : -file => $filename -format => format
format
Title : format Usage : $format = $stream->format() Function: Get the assembly format Returns : assembly format Args : none
next_assembly
Title : next_assembly Usage : $scaffold = $stream->next_assembly() Function: Reads the next assembly object from the stream and returns it. Returns : a Bio::Assembly::ScaffoldI compliant object Args : none
next_contig
Title : next_contig Usage : $contig = $stream->next_contig() Function: Reads the next contig or singlet from the stream and returns it. Returns : a Bio::Assembly::Contig or Bio::Contig::Assembly::Singlet Args : none
write_assembly
Title : write_assembly Usage : $stream->write_assembly($assembly) Function: Write the assembly object in desired format. This method calls write_header(), write_contigs() and write_footer() internally. Returns : 1 on success, 0 for error Args : A Bio::Assembly::Scaffold object
write_header
Title : write_header Usage : $stream->write_header($assembly) Function: Write the start of the assembly file. It can be called at any time, not when starting to write the assembly file. Returns : 1 on success, 0 for error Args : A Bio::Assembly::Scaffold object or ... (check the specific format driver for more details)
write_contig
Title : write_contig Usage : $stream->write_contig($contig) Function: Write a contig object in the desired format. Returns : 1 on success, 0 for error Args : A Bio::Assembly::Contig object
write_footer
Title : write_footer Usage : $stream->write_footer($assembly) Function: Write the start of the assembly file. Returns : 1 on success, 0 for error Args : A Bio::Assembly::Scaffold object or ... (check the specific format driver for more details)
_load_format_module
Title : _load_format_module Usage : *INTERNAL Assembly::IO stuff* Function: Loads up (like use) a module at run time on demand Example : Returns : Args :
_guess_format
Title : _guess_format Usage : $obj->_guess_format($filename) Function: guess format based on file suffix Example : Returns : guessed format of filename (lower case) Args : Notes : formats that _filehandle() will guess includes ace, phrap and tigr at the moment
_sort
Title : _sort Usage : @sorted_values = $ass_io->_sort(@values) Function: Sort a list of values naturally if Sort::Naturally is installed (nicer), lexically otherwise (not as nice, but safe) Returns : array of sorted values Args : array of values to sort