SYNOPSIS

use Bio::DB::SeqFeature::Store;
# Open the sequence database
my $db = Bio::DB::SeqFeature::Store->new( -adaptor => 'DBI::mysql',
-dsn => 'dbi:mysql:test');
my $segment = $db->segment('Chr1',5000=>6000);
my @features = $segment->features('mRNA','match');

DESCRIPTION

The segment object simplifies access to Bio::DB::SeqFeature store by acting as a placeholder for a region of the genome. You can replace this statement:

 @features = $db->features(-seq_id=>'Chr1',
                           -start=>5000,
                           -end=>6000,
                           -types=>['mRNA','match','repeat_region']);

with these statements:

 $segment = $db->segment('Chr1',5000=>6000);
 @features = $segment->features('mRNA','match','repeat_region');

You can also initialize a segment from an existing SeqFeature object. The range will be picked up from the SeqFeature boundaries:

 $segment = Bio::DB::SeqFeature::Segment->new($feature);        # for Bio::DB::SeqFeature
 $segment = Bio::DB::SeqFeature::Segment->new($feature,$store); # for other Bio::SeqFeatureI objects

The segment object implements the full Bio::SeqFeature::CollectionI interface, thereby allowing you to iterate over all features in the range.

PUBLIC METHODS

The following are public methods intended for external use.

new

 Title   : new
 Usage   : $segment = Bio::DB::SeqFeature::Segment->new(@options)
 Function: create a new Segment object
 Returns : A Bio::DB::SeqFeature::Segment object
 Args    : several - see below
 Status  : public

This class method creates a Bio::DB::SeqFeature::Segment object. You must provide a Bio::DB::SeqFeature::Store as well as the coordinates of the segment. These arguments can be provided explicitly or indirectly.

First form:

 $segment = Bio::DB::SeqFeature::Segment->new($store,$seqid,$start,$end,$strand)

In this form a segment is defined by a Bio::DB::SeqFeature::Store, the sequence ID, the start, end and strand. This is the form that is invoked internally by Bio::DB::SeqFeature::Store when you call its segment() method.

Second form:

 $segment = Bio::DB::SeqFeature::Segment->new($seqfeature [,$store]);

In this form, you pass new() a Bio::SeqFeatureI object. The segment is constructed from the seq_id and coordinates are taken from the object. If you pass a store-aware seqfeature object (e.g. Bio::DB::SeqFeature) then the store database is also derived from the feature. Otherwise you will have to pass the store as a second argument.

features

 Title   : features
 Usage   : @features = $segment->features(@args)
 Function: fetch seqfeatures that overlap the segment
 Returns : list of features
 Args    : see below
 Status  : Public

This is the workhorse for feature query and retrieval. It takes a series of -name=>$value arguments filter arguments. Features that match all the filters are returned.

  Argument       Value
  --------       -----
 Location filters:
  -strand        Strand
  -range_type    Type of range match ('overlaps','contains','contained_in')
 Name filters:
  -name          Name of feature (may be a glob expression)
  -aliases       If true, match aliases as well as display names
  -class         Archaic argument for backward compatibility.
                  (-class=>'Clone',-name=>'ABC123') is equivalent
                  to (-name=>'Clone:ABC123')
 Type filters:
  -types         List of feature types (array reference) or one type (scalar)
  -type          Synonym for the above
  -primary_tag   Synonym for the above
  -attributes    Hashref of attribute=>value pairs as per
                    get_features_by_attribute(). Multiple alternative values
                    can be matched by providing an array reference.
  -attribute     synonym for -attributes

This is identical to the Bio::DB::SeqFeature::Store->features() method, except that the -seq_id, -start, and -end arguments are provided by the segment object. If a simple list of arguments is provided, then the list is taken to be the set of feature types (primary tags) to filter on.

Examples:

All features that overlap the current segment:

 @features = $segment->features;

All features of type mRNA that overlap the current segment:

 @features = $segment->features('mRNA');

All features that are completely contained within the current segment:

 @features = $segment->features(-range_type=>'contains');

All ``confirmed'' mRNAs that overlap the current segment:

 @features = $segment->features(-attributes=>{confirmed=>1},-type=>'mRNA');

get_seq_stream

 Title   : get_seq_stream
 Usage   : $iterator = $segment->get_seq_stream(@args)
 Function: return an iterator across all features in the database
 Returns : a Bio::DB::SeqFeature::Store::Iterator object
 Args    : (optional) the feature() method
 Status  : public

This is identical to Bio::DB::SeqFeature::Store->get_seq_stream() except that the location filter is always automatically applied so that the iterator you receive returns features that overlap the segment's region.

When called without any arguments this method will return an iterator object that will traverse all indexed features in the database that overlap the segment's region. Call the iterator's next_seq() method to step through them (in no particular order):

  my $iterator = $db->get_seq_stream;
  while (my $feature = $iterator->next_seq) {
    print $feature->primary_tag,' ',$feature->display_name,"\n";
  }

You can select a subset of features by passing a series of filter arguments. The arguments are identical to those accepted by $segment->features().

get_feature_stream() ican be used as a synonym for this method.

store

 Title   : store
 Usage   : $store = $segment->store
 Function: return the Bio::DB::SeqFeature::Store object associated with the segment
 Returns : a Bio::DB::SeqFeature::Store: object
 Args    : none
 Status  : public

primary_tag, type,

 Title   : primary_tag,type
 Usage   : $primary_tag = $segment->primary_tag
 Function: returns the string "region"
 Returns : "region"
 Args    : none
 Status  : public

The primary_tag method returns the constant tag ``region''. type() is a synonym for this method.

as_string

 Title   : as_string
 Usage   : $name = $segment->as_string
 Function: expands the object into a human-readable string
 Returns : "seq_id:start..end"
 Args    : none
 Status  : public

The as_string() method is overloaded into the "`` operator so that the object is represented as a human readable string in the form ''seq_id:start..end" when used in a string context.

rel2abs

 Title   : rel2abs
 Usage   : @coords = $s->rel2abs(@coords)
 Function: convert relative coordinates into absolute coordinates
 Returns : a list of absolute coordinates
 Args    : a list of relative coordinates
 Status  : Public

This function takes a list of positions in relative coordinates to the segment, and converts them into absolute coordinates.

abs2rel

 Title   : abs2rel
 Usage   : @rel_coords = $s->abs2rel(@abs_coords)
 Function: convert absolute coordinates into relative coordinates
 Returns : a list of relative coordinates
 Args    : a list of absolute coordinates
 Status  : Public

This function takes a list of positions in absolute coordinates and returns a list expressed in relative coordinates.

Bio::SeqFeatureI compatibility methods

For convenience, segments are interchangeable with Bio::SeqFeature objects in many cases. This means that segments can be passed to BioPerl modules that expect Bio::SeqFeature objects and they should work as expected. The primary tag of segment objects is ``region'' (SO:0000001 ``Continous sequence >=1 base pair'').

All these methods are read-only except for the primary_id, which can be get or set.

The following Bio::SeqFeatureI methods are supported:

start

end

seq_id

strand

length

display_name

primary_id

primary_tag (always returns "region")

source_tag (always returns "Bio::DB::SeqFeature::Segment")

get_SeqFeatures (always returns an empty list)

seq

entire_seq

location

All Bio::RangeI methods

AUTHOR

Lincoln Stein <[email protected]>.

Copyright (c) 2006 Cold Spring Harbor Laboratory.

This library is free software; you can redistribute it and/or modify it under the same terms as Perl itself.