SYNOPSIS
# get a WebDBSeqI object somehow
# assuming it is a nucleotide db
my $seq = $db->get_Seq_by_id('ROA1_HUMAN')
DESCRIPTION
Provides core set of functionality for connecting to a web based database for retriving sequences.Users wishing to add another Web Based Sequence Dabatase will need to extend this class (see Bio::DB::SwissProt or Bio::DB::NCBIHelper for examples) and implement the get_request method which returns a HTTP::Request for the specified uids (accessions, ids, etc depending on what query types the database accepts).
FEEDBACK
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AUTHOR - Jason Stajich
Email < [email protected] >APPENDIX
The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _get_Seq_by_id
Title : get_Seq_by_id
Usage : $seq = $db->get_Seq_by_id('ROA1_HUMAN')
Function: Gets a Bio::Seq object by its name
Returns : a Bio::Seq object
Args : the id (as a string) of a sequence
Throws : "id does not exist" exception
get_Seq_by_acc
Title : get_Seq_by_acc
Usage : $seq = $db->get_Seq_by_acc('X77802');
Function: Gets a Bio::Seq object by accession number
Returns : A Bio::Seq object
Args : accession number (as a string)
Throws : "acc does not exist" exception
get_Seq_by_gi
Title : get_Seq_by_gi
Usage : $seq = $db->get_Seq_by_gi('405830');
Function: Gets a Bio::Seq object by gi number
Returns : A Bio::Seq object
Args : gi number (as a string)
Throws : "gi does not exist" exception
get_Seq_by_version
Title : get_Seq_by_version
Usage : $seq = $db->get_Seq_by_version('X77802.1');
Function: Gets a Bio::Seq object by sequence version
Returns : A Bio::Seq object
Args : accession.version (as a string)
Throws : "acc.version does not exist" exception
get_request
Title : get_request Usage : my $url = $self->get_request Function: returns a HTTP::Request object Returns : Args : %qualifiers = a hash of qualifiers (ids, format, etc)
get_Stream_by_id
Title : get_Stream_by_id
Usage : $stream = $db->get_Stream_by_id( [$uid1, $uid2] );
Function: Gets a series of Seq objects by unique identifiers
Returns : a Bio::SeqIO stream object
Args : $ref : a reference to an array of unique identifiers for
the desired sequence entries
get_Stream_by_acc
Title : get_Stream_by_acc
Usage : $seq = $db->get_Stream_by_acc([$acc1, $acc2]);
Function: Gets a series of Seq objects by accession numbers
Returns : a Bio::SeqIO stream object
Args : $ref : a reference to an array of accession numbers for
the desired sequence entries
Note : For GenBank, this just calls the same code for get_Stream_by_id()
get_Stream_by_gi
Title : get_Stream_by_gi
Usage : $seq = $db->get_Stream_by_gi([$gi1, $gi2]);
Function: Gets a series of Seq objects by gi numbers
Returns : a Bio::SeqIO stream object
Args : $ref : a reference to an array of gi numbers for
the desired sequence entries
Note : For GenBank, this just calls the same code for get_Stream_by_id()
get_Stream_by_version
Title : get_Stream_by_version
Usage : $seq = $db->get_Stream_by_version([$version1, $version2]);
Function: Gets a series of Seq objects by accession.versions
Returns : a Bio::SeqIO stream object
Args : $ref : a reference to an array of accession.version strings for
the desired sequence entries
Note : For GenBank, this is implemeted in NCBIHelper
get_Stream_by_query
Title : get_Stream_by_query
Usage : $stream = $db->get_Stream_by_query($query);
Function: Gets a series of Seq objects by way of a query string or oject
Returns : a Bio::SeqIO stream object
Args : $query : A string that uses the appropriate query language
for the database or a Bio::DB::QueryI object. It is suggested
that you create the Bio::DB::Query object first and interrogate
it for the entry count before you fetch a potentially large stream.
default_format
Title : default_format Usage : my $format = $self->default_format Function: Returns default sequence format for this module Returns : string Args : none
request_format
Title : request_format
Usage : my ($req_format, $ioformat) = $self->request_format;
$self->request_format("genbank");
$self->request_format("fasta");
Function: Get/Set sequence format retrieval. The get-form will normally not
be used outside of this and derived modules.
Returns : Array of two strings, the first representing the format for
retrieval, and the second specifying the corresponding SeqIO format.
Args : $format = sequence format
get_seq_stream
Title : get_seq_stream
Usage : my $seqio = $self->get_seq_stream(%qualifiers)
Function: builds a url and queries a web db
Returns : a Bio::SeqIO stream capable of producing sequence
Args : %qualifiers = a hash qualifiers that the implementing class
will process to make a url suitable for web querying
url_base_address
Title : url_base_address
Usage : my $address = $self->url_base_address or
$self->url_base_address($address)
Function: Get/Set the base URL for the Web Database
Returns : Base URL for the Web Database
Args : $address - URL for the WebDatabase
proxy
Title : proxy
Usage : $httpproxy = $db->proxy('http') or
$db->proxy(['http','ftp'], 'http://myproxy' )
Function: Get/Set a proxy for use of proxy
Returns : a string indicating the proxy
Args : $protocol : an array ref of the protocol(s) to set/get
$proxyurl : url of the proxy to use for the specified protocol
$username : username (if proxy requires authentication)
$password : password (if proxy requires authentication)
authentication
Title : authentication Usage : $db->authentication($user,$pass) Function: Get/Set authentication credentials Returns : Array of user/pass Args : Array or user/pass
retrieval_type
Title : retrieval_type
Usage : $self->retrieval_type($type);
my $type = $self->retrieval_type
Function: Get/Set a proxy for retrieval_type (pipeline, io_string or tempfile)
Returns : string representing retrieval type
Args : $value - the value to store
This setting affects how the data stream from the remote web server is processed and passed to the Bio::SeqIO layer. Three types of retrieval types are currently allowed:
pipeline Perform a fork in an attempt to begin streaming
while the data is still downloading from the remote
server. Disk, memory and speed efficient, but will
not work on Windows or MacOS 9 platforms.
io_string Store downloaded database entry(s) in memory. Can be
problematic for batch downloads because entire set
of entries must fit in memory. Alll entries must be
downloaded before processing can begin.
tempfile Store downloaded database entry(s) in a temporary file.
All entries must be downloaded before processing can
begin.
The default is pipeline, with automatic fallback to io_string if pipelining is not available.
url_params
Title : url_params
Usage : my $params = $self->url_params or
$self->url_params($params)
Function: Get/Set the URL parameters for the Web Database
Returns : url parameters for Web Database
Args : $params - parameters to be appended to the URL for the WebDatabase
ua
Title : ua
Usage : my $ua = $self->ua or
$self->ua($ua)
Function: Get/Set a LWP::UserAgent for use
Returns : reference to LWP::UserAgent Object
Args : $ua - must be a LWP::UserAgent
postprocess_data
Title : postprocess_data
Usage : $self->postprocess_data ( 'type' => 'string',
'location' => \$datastr);
Function: process downloaded data before loading into a Bio::SeqIO
Returns : void
Args : hash with two keys - 'type' can be 'string' or 'file'
- 'location' either file location or string
reference containing data
delay
Title : delay Usage : $secs = $self->delay([$secs]) Function: get/set number of seconds to delay between fetches Returns : number of seconds to delay Args : new value
NOTE: the default is to use the value specified by delay_policy(). This can be overridden by calling this method, or by passing the -delay argument to new().
delay_policy
Title : delay_policy Usage : $secs = $self->delay_policy Function: return number of seconds to delay between calls to remote db Returns : number of seconds to delay Args : none
NOTE: The default delay policy is 0s. Override in subclasses to implement delays. The timer has only second resolution, so the delay will actually be +/- 1s.
_sleep
Title : _sleep Usage : $self->_sleep Function: sleep for a number of seconds indicated by the delay policy Returns : none Args : none
NOTE: This method keeps track of the last time it was called and only imposes a sleep if it was called more recently than the delay_policy() allows.
mod_perl_api
Title : mod_perl_api Usage : $version = self->mod_perl_api Function: Returns API version of mod_perl being used based on set env. variables Returns : mod_perl API version; if mod_perl isn't loaded, returns 0 Args : none