SYNOPSIS

# Complete code for making an index for one or more GenBank files
use strict;
use Bio::Index::GenBank;
my $Index_File_Name = shift;
my $inx = Bio::Index::GenBank->new(-filename => $Index_File_Name,
-write_flag => 'WRITE');
$inx->make_index(@ARGV);
# Print out sequences present in the index in gcg format
use Bio::Index::GenBank;
use Bio::SeqIO;
use strict;
my $Index_File_Name = shift;
my $inx = Bio::Index::GenBank->new(-filename => $Index_File_Name);
my $seqio = Bio::SeqIO->new(-format => 'gcg');
foreach my $id (@ARGV) {
my $seq = $inx->fetch($id); # Returns Bio::Seq object
$seqio->write_seq($seq);
}
# alternatively
my ($locus, $acc);
my $seq1 = $inx->get_Seq_by_id($locus);
my $seq2 = $inx->get_Seq_by_acc($acc);

DESCRIPTION

By default the index that is created uses the LOCUS, ACCESSION, and VERSION identifiers as keys. Inherits functions for managing dbm files from Bio::Index::Abstract.pm, and provides the basic functionality for indexing GenBank files, and retrieving the sequence from them. For best results 'use strict'.

You can also set or customize the unique key used to retrieve by writing your own function and calling the id_parser() method. For example:

   $inx->id_parser(\&get_id);
   # make the index
   $inx->make_index($file_name);
   # here is where the retrieval key is specified
   sub get_id {
      my $line = shift;
      $line =~ /clone="(\S+)"/;
      $1;
   }

FEED_BACK

Mailing Lists

User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to one of the Bioperl mailing lists. Your participation is much appreciated.

  [email protected]                  - General discussion
  http://bioperl.org/wiki/Mailing_lists  - About the mailing lists

Support

Please direct usage questions or support issues to the mailing list:

[email protected]

rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible.

Reporting Bugs

Report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via the web:

  https://github.com/bioperl/bioperl-live/issues

AUTHOR - Ewan Birney

Email - [email protected]

APPENDIX

The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _

_index_file

  Title   : _index_file
  Usage   : $index->_index_file($file_name, $i)
  Function: Specialized function to index GenBank format files.
            Is provided with a filename and an integer
            by make_index in its SUPER class.
  Example : 
  Returns : 
  Args    :

id_parser

  Title   : id_parser
  Usage   : $index->id_parser( CODE )
  Function: Stores or returns the code used by record_id to
            parse the ID for record from a string.
            Returns \&default_id_parser (see below) if not
            set. An entry will be added to
            the index for each string in the list returned.
  Example : $index->id_parser( \&my_id_parser )
  Returns : reference to CODE if called without arguments
  Args    : CODE

default_id_parser

  Title   : default_id_parser
  Usage   : $id = default_id_parser($line)
  Function: The default parser for GenBank.pm
  Returns : Array of specified ids
  Args    : a line string

_file_format

 Title   : _file_format
 Usage   : Internal function for indexing system
 Function: Provides file format for this database
 Example :
 Returns : 
 Args    :