SYNOPSIS
#documentation needed
DESCRIPTION
This is used as possible parent for aminoacid range object classes. Or it can be used straight away to define aminoacid ranges. The idea is that the ranges defined are attached to a Translation object and they refer to its coordinate-system when they are first created (via the new() method). When they are created they are anyway linked to the underlying DNA LiveSeq by way of the LiveSeq labels. This allows to preserve the ranges even if the numbering changes in the Translation due to deletions or insertions.The protein sequence associated with the AARange can be accessed via the usual seq() or subseq() methods.
The start and end of the AARange in protein coordinate system can be fetched with aa_start() and aa_end() methods. Note: the behaviour of these methods would be influenced by the coordinate_start set in the corresponding Translation object. This can be desirable but can also lead to confusion if the coordinate_start had been changed and the original position of the AARange was to be retrieved.
start() and end() methods of the AARange will point to the labels identifying the first nucleotide of the first and last triplet coding for the start and end of the AminoAcidRange.
The underlying nucleotide sequence of the AARange can be retrieved with the labelsubseq() method. This would retrieve the whole DNA sequence, including possible introns. This is called ``DNA_sequence''.
To fetch the nucleotide sequence of the Transcript, without introns, the labelsubseq() of the attached Transcript (the Transcript the Translation comes from) has to be accessed. This is called ``cDNA_sequence''.
Here are the operations to retrieve these latter two kinds of sequences:
$startlabel=$AARange->start;
$endtripletlabel=$AARange->end;
$endlabel=$AARange->{'seq'}->label(3,$endtripletlabel,$AARange->strand);
$dnaseq=$AARange->labelsubseq($startlabel,undef,$endlabel));
$cdnaseq=$AARange->get_Transcript->labelsubseq($startlabel,undef,$endlabel);
To simplify, these operations have been included in two additional methods: dna_seq() and cdna_seq().
These would return the whole sequence, as in the examples above. But the above general scheme can be used by specifying different labels, to retrieve hypothetical subsequences of interest.
APPENDIX
The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _new
Title : new
Usage : $aarange = Bio::LiveSeq::AARange->new(-translation => $obj_ref,
-start => $beginaa,
-end => $endaa,
-name => "ABCD",
-description => "DCBA",
-translength => $length);
Function: generates a new AminoAcidRange LiveSeq object
Returns : reference to a new object of class AARange
Errorcode -1
Args : two positions in AminoAcid coordinate numbering
an object reference specifying to which translation the aminoacid
ranges refer to
a name and a description (optional)
an optional "translength" argument: this can be given when
a lot of AARanges are to be created at the same time for the same
Translation object, calculating it with $translation->length
This would increase the speed, avoiding the new() function to
calculate everytime the same length again and again for every obj.
get_Transcript
Title : valid
Usage : $transcript = $obj->get_Transcript()
Function: retrieves the reference to the object of class Transcript (if any)
attached to a LiveSeq object
Returns : object reference
Args : none
get_Translation
Title : valid
Usage : $translation = $obj->get_Translation()
Function: retrieves the reference to the object of class Translation (if any)
attached to a LiveSeq object
Returns : object reference
Args : none
aa_start
Title : aa_start
Usage : $end = $aarange->aa_start()
Returns : integer (position, according to Translation coordinate system) of
the start of an AminoAcidRange object
Args : none
aa_end
Title : aa_end
Usage : $end = $aarange->aa_end()
Returns : integer (position, according to Translation coordinate system) of
the end of an AminoAcidRange object
Args : none
dna_seq
Title : dna_seq
Usage : $end = $aarange->dna_seq()
Returns : the sequence at DNA level of the entire AminoAcidRange
this would include introns (if present)
Args : none
cdna_seq
Title : cdna_seq
Usage : $end = $aarange->cdna_seq()
Returns : the sequence at cDNA level of the entire AminoAcidRange
i.e. this is the part of the Transcript that codes for the
AminoAcidRange. It would be composed just of exonic DNA.
Args : none