$in = Bio::Restriction::IO->new(-file => "inputfilename" ,
-format => 'withrefm');
my $res = $in->read; # a Bio::Restriction::EnzymeCollection
DESCRIPTIONBio::Restriction::IO is a handler module for the formats in the Restriction IO set, e.g. "Bio::Restriction::IO::xxx". It is the officially sanctioned way of getting at the format objects, which most people should use.
The structure, conventions and most of the code is inherited from Bio::SeqIO. The main difference is that instead of using methods "next_seq", you drop "_seq" from the method name.
Also, instead of dealing only with individual Bio::Restriction::Enzyme objects, "read()" will slurp in all enzymes into a Bio::Restriction::EnzymeCollection object.
For more details, see documentation in Bio::SeqIO.
TO DOAt the moment, these can be use mainly to get a custom set if enzymes in "withrefm" or "itype2" formats into Bio::Restriction::Enzyme or Bio::Restriction::EnzymeCollection objects. Using "bairoch" format is highly experimental and is not recommmended at this time.
This class inherits from Bio::SeqIO for convenience sake, though this should inherit from Bio::Root::Root. Get rid of Bio::SeqIO inheritance by copying relevant methods in.
"write()" methods are currently not implemented for any format except "base". Using "write()" even with "base" format is not recommended as it does not support multicut/multisite enzyme output.
Should additional formats be supported (such as XML)?
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AUTHORRob Edwards, [email protected]
CONTRIBUTORSHeikki Lehvaslaiho, heikki-at-bioperl-dot-org
APPENDIXThe rest of the documentation details each of the object methods. Internal methods are usually preceded with a _
Title : new Usage : $stream = Bio::Restriction::IO->new(-file => $filename, -format => 'Format') Function: Returns a new seqstream Returns : A Bio::Restriction::IO::Handler initialised with the appropriate format Args : -file => $filename -format => format -fh => filehandle to attach to
Title : format Usage : $format = $stream->format() Function: Get the restriction format Returns : restriction format Args : none
Title : read Usage : $renzs = $stream->read Function: reads all the restrction enzymes from the stream Returns : a Bio::Restriction::EnzymeCollection object Args :
Title : write Usage : $stream->write($seq) Function: writes the $seq object into the stream Returns : 1 for success and 0 for error Args : Bio::Restriction::EnzymeCollection object
Title : _guess_format Usage : $obj->_guess_format($filename) Function: Example : Returns : guessed format of filename (lower case) Args :