SYNOPSIS
use Bio::Search::Hit::BlastHit;
my $hit = Bio::Search::Hit::BlastHit->new(-algorithm => 'blastp');
# See Bio::Search::Hit::GenericHit for information about working with Hits.
# TODO: Describe how to configure a SearchIO stream so that it generates # GenericHit objects.
DESCRIPTION
This object is a subclass of Bio::Search::Hit::GenericHit and provides some operations that facilitate working with BLAST and PSI-BLAST Hits.For general information about working with Hits, see Bio::Search::Hit::GenericHit.
FEEDBACK
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Reporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track of the bugs and their resolution. Bug reports can be submitted via the web:
https://github.com/bioperl/bioperl-live/issues
APPENDIX
The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _new
Title : new Usage : my $obj = Bio::Search::Hit::GenericHit->new(); Function: Builds a new Bio::Search::Hit::GenericHit object Returns : Bio::Search::Hit::GenericHit Args : See Bio::Search::Hit::GenericHit() for other args. Here are the BLAST-specific args that can be used when creating BlastHit objects: -iteration => integer for the PSI-Blast iteration number -found_again => boolean, true if hit appears in a "previously found" section of a PSI-Blast report.
iteration
Usage : $hit->iteration( $iteration_num ); Purpose : Gets the iteration number in which the Hit was found. Example : $iteration_num = $sbjct->iteration(); Returns : Integer greater than or equal to 1 Non-PSI-BLAST reports will report iteration as 1, but this number is only meaningful for PSI-BLAST reports. Argument : iteration_num (optional, used when setting only) Throws : none
See Also : found_again()
found_again
Title : found_again Usage : $hit->found_again; $hit->found_again(1); Purpose : Gets a boolean indicator whether or not the hit has been found in a previous iteration. This is only applicable to PSI-BLAST reports. This method indicates if the hit was reported in the "Sequences used in model and found again" section of the PSI-BLAST report or if it was reported in the "Sequences not found previously or not previously below threshold" section of the PSI-BLAST report. Only for hits in iteration > 1. Example : if( $hit->found_again()) { ... }; Returns : Boolean, true (1) if the hit has been found in a previous PSI-BLAST iteration. Returns false (0 or undef) for hits that have not occurred in a previous PSI-BLAST iteration. Argument : Boolean (1 or 0). Only used for setting. Throws : none
See Also : iteration()