SYNOPSIS
use Bio::SearchIO;
my $in = Bio::SearchIO->new(-file => 'result.blast',
-format => 'blast');
my $out = Bio::SearchIO->new(-output_format => 'GbrowseGFF',
-output_cigar => 1,
-output_signif => 1,
-file => ">result.gff");
while( my $r = $in->next_result ) {
$out->write_result($r);
}
DESCRIPTION
This writer produces Gbrowse flavour GFF from a Search::Result object.AUTHOR Mark Wilkinson
Email markw-at-illuminae-dot-comCONTRIBUTORS
Susan Miller sjmiller at email-DOT-arizon-DOT-edu Jason Stajich jason at bioperl-dot-orgFEEDBACK
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APPENDIX
The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _new
Title : new Usage : my $obj = Bio::SearchIO::Writer::GbrowseGFF->new(@args); Function: Builds a new Bio::SearchIO::Writer::GbrowseGFF object Returns : an instance of Bio::SearchIO::Writer::GbrowseGFF Args : -e_value => 10 : set e_value parsing cutoff (default undef) (note the -e_value flag is deprecated.)
to_string
Purpose : Produce the Gbrowse format GFF lines for a Result Usage : print $writer->to_string( $result_obj, @args); Argument : $result_obj = A Bio::Search::Result::ResultI object -version => 1|2|2.5|3 ; the GFF format you want to output (default 3) -match_tag => match|cDNA_match|EST_match|translated_nucleotide_match nucleotide_to_protein_match|nucleotide_motif This is the SO term to be placed in GFF column 3. -prefix => String to prefix the group by, default is EST (see %Defaults class variable) A default can also be set on object init Returns : String containing data for each search Result or any of its : sub-objects (Hits and HSPs). Throws : n/a
start_report
Title : start_report Usage : $self->start_report() Function: has no function, returns nothing Returns : empty string Args : none
end_report
Title : end_report Usage : $self->end_report() Function: has no function, returns nothing Returns : empty string Args : none
filter
Title : filter Usage : $writer->filter('hsp', \&hsp_filter); Function: Filter out either at HSP,Hit,or Result level Returns : none Args : string => data type, CODE reference Note : GbrowseGFF.pm makes no changes to the default filter code