SYNOPSIS
$obj = Bio::Seq::EncodedSeq->new( -seq => $dna,
-encoding => "CCCCCCCIIIIICCCCC",
-start => 1,
-strand => 1,
-length => 17 );
# splice out (and possibly revcomp) the coding sequence
$cds = obj->cds;
# obtain the protein translation of the sequence
$prot = $obj->translate;
# other access/inspection routines as with Bio::LocatableSeq and
# Bio::SeqI; note that coordinates are relative only to the DNA
# sequence, not it's implicit encoded protein sequence.
DESCRIPTION
Bio::Seq::EncodedSeq is a Bio::LocatableSeq object that holds a DNA sequence as well as information about the coding potential of that DNA sequence. It is meant to be useful in an alignment context, where the DNA may contain frameshifts, gaps and/or introns, or in describing the transcript of a gene. An EncodedSeq provides the ability to access the ``spliced'' coding sequence, meaning that all introns and gaps are removed, and any frameshifts are adjusted to provide a ``clean'' CDS.In order to make simultaneous use of either the DNA or the implicit encoded protein sequence coordinates, please see Bio::Coordinate::EncodingPair.
ENCODING
We use the term ``encoding'' here to refer to the series of symbols that we use to identify which residues of a DNA sequence are protein-coding (i.e. part of a codon), intronic, part of a 5' or 3', frameshift ``mutations'', etc. From this information, a Bio::Seq::EncodedSeq is able to ``figure out'' its translational CDS. There are two sets of coding characters, one termed ``implicit'' and one termed ``explicit''.The ``implicit'' encoding is a bit simpler than the ``explicit'' encoding: 'C' is used for any nucleotide that's part of a codon, 'U' for any UTR, etc. The full list is shown below:
Code Meaning ---- ------- C coding I intronic U untranslated G gapped (for use in alignments) F a "forward", +1 frameshift B a "backward", -1 frameshift
The ``explicit'' encoding is just an expansion of the ``implicit'' encoding, to denote phase:
Code Meaning ---- ------- C coding, 1st codon position D coding, 2nd codon position E coding, 3rd codon position I intronic, phase 0 (relative to intron begin) J intronic, phase 1 K intronic, phase 2 U untranslated 3'UTR V untranslated 5'UTR G gapped (for use in alignments) F a "forward", +1 frameshift B a "backward", -1 frameshift
Note that the explicit coding is meant to provide easy access to position/phase specific nucleotides:
$obj = Bio::Seq::EncodedSeq->new(-seq => "ACAATCAGACTACG...",
-encoding => "CCCCCCIII..."
);
# fetch arrays of nucleotides at each codon position:
my @pos1 = $obj->dnaseq(encoding => 'C', explicit => 1);
my @pos2 = $obj->dnaseq(encoding => 'D');
my @pos3 = $obj->dnaseq(encoding => 'E');
# fetch arrays of "3-1" codon dinucleotides, useful for genomic
# signature analyses without compounding influences of codon bias:
my @pairs = $obj->dnaseq(encoding => 'EC');
FEEDBACK
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Support
Please direct usage questions or support issues to the mailing list:rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible.
Reporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via the web:
https://github.com/bioperl/bioperl-live/issues
AUTHOR - Aaron Mackey
Email [email protected]APPENDIX
The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _new
Title : new
Usage : $obj = Bio::Seq::EncodedSeq->new(-seq => "AGTACGTGTCATG",
-encoding => "CCCCCCFCCCCCC",
-id => "myseq",
-start => 1,
-end => 13,
-strand => 1
);
Function: creates a new Bio::Seq::EncodedSeq object from a supplied DNA
sequence
Returns : a new Bio::Seq::EncodedSeq object
Args : seq - primary nucleotide sequence used to encode the
protein; note that any positions involved in a
gap ('G') or backward frameshift ('B') should
have one or more gap characters; if the encoding
specifies G or B, but no (or not enough) gap
characters exist, *they'll be added*; similary,
if there are gap characters without a
corresponding G or B encoding, G's will be
inserted into the encoding. This allows some
flexibility in specifying your sequence and
coding without having to calculate a lot of the
encoding for yourself.
encoding - a string of characters (see Encoding Table)
describing backwards frameshifts implied by the
encoding but not present in the sequence will be
added (as '-'s) to the sequence. If not
supplied, it will be assumed that all positions
are coding (C). Encoding may include either
implicit phase encoding characters (i.e. "CCC")
and/or explicit encoding characters (i.e. "CDE").
Additionally, prefixed numbers may be used to
denote repetition (i.e. "27C3I28C").
Alternatively, encoding may be a hashref
datastructure, with encoding characters as keys
and Bio::LocationI objects (or arrayrefs of
Bio::LocationI objects) as values, e.g.:
{ C => [ Bio::Location::Simple->new(1,9),
Bio::Location::Simple->new(11,13) ],
F => Bio::Location::Simple->new(10,10),
} # same as "CCCCCCCCCFCCC"
Note that if the location ranges overlap, the
behavior of the encoding will be undefined
(well, it will be defined, but only according to
the order in which the hash keys are read, which
is basically undefined ... just don't do that).
id, start, end, strand - as with Bio::LocatableSeq; note
that the coordinates are relative to the
encoding DNA sequence, not the implicit protein
sequence. If strand is reversed, then the
encoding is assumed to be relative to the
reverse strand as well.
encoding
Title : encoding
Usage : $obj->encoding("CCCCCC");
$obj->encoding( -encoding => { I => $location } );
$enc = $obj->encoding(-explicit => 1);
$enc = $obj->encoding("CCCCCC", -explicit => 1);
$enc = $obj->encoding(-location => $location,
-explicit => 1,
-absolute => 1 );
Function: get/set the objects encoding, either globally or by location(s).
Returns : the (possibly new) encoding string.
Args : encoding - see the encoding argument to the new() function.
explicit - whether or not to return explicit phase
information in the coding (i.e. "CCC" becomes
"CDE", "III" becomes "IJK", etc); defaults to 0.
location - optional; location to get/set the encoding.
Defaults to the entire sequence.
absolute - whether or not the locational elements (either
in the encoding hashref or the location
argument) are relative to the absolute start/end
of the Bio::LocatableSeq, or to the internal,
relative coordinate system (beginning at 1);
defaults to 0 (i.e. relative)
cds
Title : cds
Usage : $cds = $obj->cds(-nogaps => 1);
Function: obtain the "spliced" DNA sequence, by removing any
nucleotides that participate in an UTR, forward frameshift
or intron, and replacing any unknown nucleotide implied by
a backward frameshift or gap with N's.
Returns : a Bio::Seq::EncodedSeq object, with an encoding consisting only
of "CCCC..".
Args : nogaps - strip any gap characters (resulting from 'G' or 'B'
encodings), rather than replacing them with N's.
translate
Title : translate
Usage : $prot = $obj->translate(@args);
Function: obtain the protein sequence encoded by the underlying DNA
sequence; same as $obj->cds()->translate(@args).
Returns : a Bio::PrimarySeq object.
Args : same as the translate() function of Bio::PrimarySeqI
protseq
Title : seq
Usage : $protseq = $obj->protseq();
Function: obtain the raw protein sequence encoded by the underlying
DNA sequence; This is the same as calling
$obj->translate()->seq();
Returns : a string of single-letter amino acid codes
Args : same as the seq() function of Bio::PrimarySeq; note that this
function may not be used to set the protein sequence; see
the dnaseq() function for that.
dnaseq
Title : dnaseq
Usage : $dnaseq = $obj->dnaseq();
$obj->dnaseq("ACGTGTCGT", "CCCCCCCCC");
$obj->dnaseq(-seq => "ATG",
-encoding => "CCC",
-location => $loc );
@introns = $obj->$dnaseq(-encoding => 'I')
Function: get/set the underlying DNA sequence; will overwrite any
current DNA and/or encoding information present.
Returns : a string of single-letter nucleotide codes, including any
gaps implied by the encoding.
Args : seq - the DNA sequence to be used as a replacement
encoding - the encoding of the DNA sequence (see the new()
constructor); defaults to all 'C' if setting a
new DNA sequence. If no new DNA sequence is
being provided, then the encoding is used as a
"filter" for which to return fragments of
non-overlapping DNA that match the encoding.
location - optional, the location of the DNA sequence to
get/set; defaults to the entire sequence.