SYNOPSIS
use Bio::SeqIO;
use Bio::SeqFeature::Tools::FeatureNamer;
# first fetch a genbank SeqI object
$seqio =
Bio::SeqIO->new(-file=>'AE003644.gbk',
-format=>'GenBank');
$seq = $seqio->next_seq();
$namer = Bio::SeqFeature::Tools::FeatureNamer->new;
my @features = $seq->get_SeqFeatures;
foreach my $feature (@features) {
$namer->name_feature($feature) unless $feature->display_name;
}
DESCRIPTION
This is a helper class for providing names for SeqFeaturesThe Bio::SeqFeatureI class provides a display_name method. Typically the display_name is not set when parsing formats such as genbank - instead properties such as label, product or gene are set in a somewhat inconsistent manner.
In addition, when generating subfeatures (for example, exons that are subfeatures of a transcript feature), it is often desirable to name these subfeatures before either exporting to another format or reporting to the user.
This module is intended to help given uniform display_names to features and their subfeatures.
TODO
Currently the naming policy is hardcoded. It may be desirable to allow plugging in variations on naming policies; this could be done either by subclassing, anonymous subroutines (closures) or parameterization. Contact the author if you feel you have need for a different naming policyFEEDBACK
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AUTHOR - Chris Mungall
Email: cjm AT fruitfly DOT orgAPPENDIX
The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _new
Title : new Usage : $unflattener = Bio::SeqFeature::Tools::FeatureNamer->new(); Function: constructor Example : Returns : a new Bio::SeqFeature::Tools::FeatureNamer Args : see below
name_feature
Title : name_feature Usage : $namer->name_feature($sf); Function: sets display_name Example : Returns : Args : L<Bio::SeqFeatureI>
This method calls generate_feature_name() and uses the returned value to set the display_name of the feature
name_contained_features
Title : name_contained_features Usage : $namer->name_contained_features($sf); Function: sets display_name for all features contained by sf Example : Returns : Args : L<Bio::SeqFeatureI>
iterates through all subfeatures of a certain feature (using get_all_SeqFeatures) and names each subfeatures, based on the generated name for the holder feature
A subfeature is named by concatenating the generated name of the container feature with the type and a number.
For example, if the containing feature is a gene with display name dpp, subfeatures will be named dpp-mRNA-1 dpp-mRNA2 dpp-exon1 dpp-exon2 etc
generate_feature_name
Title : generate_feature_name Usage : $name = $namer->generate_feature_name($sf); Function: derives a sensible human readable name for a $sf Example : Returns : str Args : L<Bio::SeqFeatureI>
returns a generated name (but does not actually set display_name).
If display_name is already set, the method will return this
Otherwise, the name will depend on the property:
- label
- product
- gene
- locus_tag
(in order of priority)