SYNOPSIS

use Bio::Tools::Primer::Feature;
my $pf = Bio::Tools::Primer::Feature->new( -start => $start, -end => $end, -strand => $strand);
$pf->attach_seq($seq);
# is a SeqFeatureI
print "primer starts at ",$pf->start," with sequence ",$pf->seq->seq(),"\n";
# helper functions
print "GC percentage ",$pf->gc(),"\n";
print "has inversion of size 4 at ",$pf->inversion(4),"\n";

DESCRIPTION

Primer Features represents one primer in a primer pair. This object is mainly for designing primers, and probably principly used in the primer design system

FEEDBACK

Mailing Lists

User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing list. Your participation is much appreciated.

  [email protected]                  - General discussion
  http://bioperl.org/wiki/Mailing_lists  - About the mailing lists

Support

Please direct usage questions or support issues to the mailing list:

[email protected]

rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible.

Reporting Bugs

Report bugs to the Bioperl bug tracking system to help us keep track of the bugs and their resolution. Bug reports can be submitted via the web:

  https://github.com/bioperl/bioperl-live/issues

AUTHOR - Ewan Birney

Email birney-at-ebi.ac.uk

APPENDIX

The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _