SYNOPSIS
#run
my @params = (
trset => 'BL.erpin',
region => [1, 10],
# Set up search strategy this way...
strategy => [ 'umask' => [1, 2],
'umask' => [1, 2, 3, 4],
'umask' => [1, 2, 3, 4, 5, 6],
'nomask',
'cutoff' => [0, 10, 15, 20]
]
# or use a simple string...
#strategy => 'D-umask 4 D-add 5 -nomask -cutoff 0 10 15',
pcw => 100
);
my $factory = Bio::Tools::Run::ERPIN->new(-program =>'erpin',
@params);
# Pass the factory a Bio::Seq object or a file name
# Returns a Bio::SearchIO object
#my $search = $factory->run("B_sub.fas");
my $search = $factory->run($seq);
my @feat;
while (my $result = $searchio->next_result){
while(my $hit = $result->next_hit){
while (my $hsp = $hit->next_hsp){
print join("\t", ( $r->query_name,
$hit->name,
$hsp->hit->start,
$hsp->hit->end,
$hsp->meta,
$hsp->score,
)), "\n";
}
}
}
DESCRIPTION
FEEDBACK
Mailing Lists
User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to one of the Bioperl mailing lists. Your participation is much appreciated.
[email protected] - General discussion http://bioperl.org/wiki/Mailing_lists - About the mailing lists
Support
Please direct usage questions or support issues to the mailing list:rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible.
Reporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via the web:
http://redmine.open-bio.org/projects/bioperl/
AUTHOR - Chris Fields
Email: cjfields-at-uiuc-dot-edu
CONTRIBUTORS
cjfields-at-uiuc-dot-edu
APPENDIX
The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _new
Title : new Usage : my $wrapper = Bio::Tools::Run::RNAMotif->new(@params) Function: creates a new RNAMotif factory Returns: Bio::Tools::Run::RNAMotif Args : list of parameters -tempfile => set tempfile flag (default 0) -outfile_name => set file to send output to (default none)
program_name
Title : program_name Usage : $factory>program_name() Function: holds the program name Returns: string Args : None
program_dir
Title : program_dir Usage : $factory->program_dir(@params) Function: returns the program directory, obtained from ENV variable. Returns: string Args :
version
Title : version Usage : $v = $prog->version(); Function: Determine the version number of the program Example : Returns : float or undef Args : none
run
Title : run Usage : $obj->run($seqFile) Function: Runs ERPIN programs and returns Bio::SearchIO Returns : Args : Must pass Bio::PrimarySeqI's or file names
tempfile
Title : tempfile Usage : $obj->tempfile(1) Function: Set tempfile flag. When set, writes output to a tempfile; this is overridden by outfile_name() if set Returns : Boolean setting (or undef if not set) Args : [OPTIONAL] Boolean
_run
Title : _run Usage : $obj->_run() Function: Internal(not to be used directly) Returns : Args :
_setparams
Title : _setparams Usage : Internal function, not to be called directly Function: creates a string of params to be used in the command string Example : Returns : string of params Args :
_writeSeqFile
Title : _writeSeqFile Usage : obj->_writeSeqFile($seq) Function: Internal(not to be used directly) Returns : writes passed Seq objects to tempfile, to be used as input for program Args :
POD ERRORS
Hey! The above document had some coding errors, which are explained below:- Around line 35:
-
Non-ASCII character seen before =encoding in ''D-umask'. Assuming ISO8859-1