Bio::Tools::Run::Newbler(3) Wrapper for local execution of Newbler


use Bio::Tools::Run::Newbler;
# Run Minmo using an input FASTA file
my $factory = Bio::Tools::Run::Newbler->new( -minimum_overlap_length => 35 );
my $asm_obj = $factory->run($fasta_file, $qual_file);
# An assembly object is returned by default
for my $contig ($assembly->all_contigs) {
... do something ...
# Read some sequences
use Bio::SeqIO;
my $sio = Bio::SeqIO->new(-file => $fasta_file, -format => 'fasta');
my @seqs;
while (my $seq = $sio->next_seq()) {
push @seqs,$seq;
# Run Newbler using input sequence objects and returning an assembly file
my $asm_file = 'results.ace';


  Wrapper module for the local execution of the proprietary DNA assembly
  program GS De Novo Assembler (Newbler) from Roche/454 v2.0.00.20:


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rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible.

Reporting Bugs

Report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via the web:

AUTHOR - Florent E Angly

 Email: florent-dot-angly-at-gmail-dot-com


The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _


 Title   : new
 Usage   : $assembler->new( -min_len   => 50,
                            -min_ident => 95 );
 Function: Creates a Newbler factory
 Returns : A Bio::Tools::Run::Newbler object
 Args    : Newbler options available in this module (from the Newbler manual):
 large                Shortcut some of the computationally expensive algorithms
                        to save some time. Useful for large or complex datasets
                        (default: off).
 ace_raw              Output the full "raw" read sequence (default: off).
 ace_trimmed          Output only the "trimmed" sequences (after low quality,
                        vector and key trimming) (default: on).
 expected_depth       Expected depth of the assembly. Filters out random-chance
                        level events at bigger depths. 0 means to not use the
                        expected depth information (default: 0).
 mid_conf_file        MID configuration file for decoding the multiplex data.
 no_trim              Disable the quality and primer trimming of the input
                        sequences (default: off).
 vector_trim          Specify a vector trimming database (in FASTA format) to
                        trim the ends of input sequences.
 vector_screen        Specify a vector screening database (in FASTA format) to
                        remove contaminants, i.e. input reads that align
                        against a sequence in the database.
 aln_identity_score   Set the alignment identity score. When multiple alignments
                        are found, it is the per-overlap column identity score
                        used to sort the overlaps for use in the progressive
                        alignment (default: 2).
 aln_difference_score Set the alignment difference score. For multiple alignments
                        this is the per-overlap difference score used to sort the
                        overlaps for use in the progressive multi-alignment
                        (default: -3).
 in_memory            Keep all sequence data in memory throughout the computation.
                        Can speed up the computation but requires more computer
                        memory (default: off).
 min_ovl_identity / minimum_overlap_similarity
                      Minimum overlap identity, i.e. the minimum percent identity
                        of overlaps used by the assembler (default: 40).
 min_ovl_length / minimum_overlap_length
                      Minimum overlap length, i.e. the minimum length of overlaps
                        considered by the assembler (default: 90). Warning: It
                        seems like this parameter is not respected by the program
                        in the current version
 no_auto_rescore      Do not use the quality score re-scoring algorithm (default:
 seed_count           Set the seed count parameter, the number of seeds required
                        in a window before an extension is made (default: 1).
 seed_length          Set the seed length parameter, i.e. the number of bases
                        between seed generation locations used in the exact
                        k-mer matching part of the overlap detection (between 6
                        16) (default: 16).
 seed_step            Set the seed step parameter, i.e. the number of bases used
                        for each seed in the exact k-mer matching part of the
                        overlap detection (i.e. the "k" value) (default: 12).
 no_duplicates        Treat each read as a separate read and do not group them
                        into duplicates for assembly or consensus calling
                       (default: off).


 Title   : _check_sequence_input
 Usage   : $assembler->_check_sequence_input($seqs)
 Function: Check that the sequence input is arrayref of sequence objects or
           a FASTA file, or a MIDinfo + dir, or a MIDinfo + file. If not, an
           error is thrown.
 Returns : 1 if the check passed
 Args    : sequence input


 Title   : out_type
 Usage   : $factory->out_type('Bio::Assembly::ScaffoldI')
 Function: Get/set the desired type of output
 Returns : The type of results to return
 Args    : Desired type of results to return (optional):
                 'Bio::Assembly::IO' object
                 'Bio::Assembly::ScaffoldI' object (default)
                 The name of a file to save the results in


 Title   :   run
 Usage   :   $factory->run($fasta_file);
 Function:   Run TIGR Assembler
 Returns :   - a Bio::Assembly::ScaffoldI object, a Bio::Assembly::IO
               object, a filename, or undef if all sequences were too small to
               be usable
 Returns :   Assembly results (file, IO object or assembly object)
 Args    :   Sequence input can be:
               * a sequence object arrayref
               * a FASTA file
               * a SFF file and optional MID information. Example:
                   [email protected]/home/xxx/myreads.sff
               * the path to an run analysis directory and MID information
             The reads must be between 50 and 2000 bp. Newbler does not support
               for input quality files. See the Newbler manual for details.


 Title   :   _run
 Usage   :   $factory->_run()
 Function:   Make a system call and run TIGR Assembler
 Returns :   An assembly file
 Args    :   - FASTA file, SFF file and MID, or analysis dir and MID
             - optional QUAL file