Bio::Tools::Run::Phylo::Hyphy::FEL(3) Wrapper around the Hyphy FEL analysis


use Bio::Tools::Run::Phylo::Hyphy::FEL;
use Bio::AlignIO;
use Bio::TreeIO;
my $alignio = Bio::AlignIO->new(-format => 'fasta',
-file => 't/data/hyphy1.fasta');
my $aln = $alignio->next_aln;
my $treeio = Bio::TreeIO->new(
-format => 'newick', -file => 't/data/hyphy1.tree');
my $fel = Bio::Tools::Run::Phylo::Hyphy::FEL->new();
my ($rc,$results) = $fel->run();


This is a wrapper around the FEL analysis of HyPhy ([Hy]pothesis Testing Using [Phy]logenies) package of Sergei Kosakowsky Pond, Spencer V. Muse, Simon D.W. Frost and Art Poon. See for more information.

This module will generate the correct list of options for interfacing with TemplateBatchFiles/Ghostrides/


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Please direct usage questions or support issues to the mailing list:

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rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible.

Reporting Bugs

Report bugs to the Bioperl bug tracking system to help us keep track of the bugs and their resolution. Bug reports can be submitted via the web:

AUTHOR - Albert Vilella

Email avilella-at-gmail-dot-com


Additional contributors names and emails here


The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _

Default Values

Valid and default values for FEL are listed below. The default values are always the first one listed. These descriptions are essentially lifted from the python wrapper or provided by the author.



 Title   : program_name
 Usage   : $factory->program_name()
 Function: holds the program name
 Returns:  string
 Args    : None


 Title   : program_dir
 Usage   : ->program_dir()
 Function: returns the program directory, obtained from ENV variable.
 Returns:  string
 Args    :


 Title   : new
 Usage   : my $obj = Bio::Tools::Run::Phylo::Hyphy::FEL->new();
 Function: Builds a new Bio::Tools::Run::Phylo::Hyphy::FEL object 
 Returns : Bio::Tools::Run::Phylo::Hyphy::FEL
 Args    : -alignment => the Bio::Align::AlignI object
           -save_tempfiles => boolean to save the generated tempfiles and
                              NOT cleanup after onesself (default FALSE)
           -tree => the Bio::Tree::TreeI object
           -params => a hashref of parameters (all passed to set_parameter)
           -executable => where the hyphy executable resides

See also: Bio::Tree::TreeI, Bio::Align::AlignI


 Title   : run
 Usage   : my ($rc,$results) = $fel->run($aln);
 Function: run the fel analysis using the default or updated parameters
           the alignment parameter must have been set
 Returns : Return code, Hash
 Args    : L<Bio::Align::AlignI> object,
           L<Bio::Tree::TreeI> object [optional]


 Title   : create_wrapper
 Usage   : $self->create_wrapper
 Function: It will create the wrapper file that interfaces with the analysis bf file
 Example :
 Returns : 
 Args    :


 Title   : error_string
 Usage   : $obj->error_string($newval)
 Function: Where the output from the last analysus run is stored.
 Returns : value of error_string
 Args    : newvalue (optional)


 Title   : set_default_parameters
 Usage   : $fel->set_default_parameters(0);
 Function: (Re)set the default parameters from the defaults
           (the first value in each array in the 
            %VALIDVALUES class variable)
 Returns : none
 Args    : boolean: keep existing parameter values

Bio::Tools::Run::WrapperBase methods


 Title   : no_param_checks
 Usage   : $obj->no_param_checks($newval)
 Function: Boolean flag as to whether or not we should
           trust the sanity checks for parameter values  
 Returns : value of no_param_checks
 Args    : newvalue (optional)


 Title   : save_tempfiles
 Usage   : $obj->save_tempfiles($newval)
 Returns : value of save_tempfiles
 Args    : newvalue (optional)


 Title   : tempdir
 Usage   : my $tmpdir = $self->tempdir();
 Function: Retrieve a temporary directory name (which is created)
 Returns : string which is the name of the temporary directory
 Args    : none


 Title   : cleanup
 Usage   : $fel->cleanup();
 Function: Will cleanup the tempdir directory after a run
 Returns : none
 Args    : none


 Title   : io
 Usage   : $obj->io($newval)
 Function:  Gets a L<Bio::Root::IO> object
 Returns : L<Bio::Root::IO>
 Args    : none