SYNOPSIS

$rnachange = Bio::Variation::RNAChange->new
('-start' => $start,
'-end' => $end,
'-length' => $len,
'-codon_pos' => $cp,
'-upStreamSeq' => $upflank,
'-dnStreamSeq' => $dnflank,
'-proof' => $proof,
'-isMutation' => 1,
'-mut_number' => $mut_number
);
$a1 = Bio::Variation::Allele->new;
$a1->seq('a');
$rnachange->allele_ori($a1);
my $a2 = Bio::Variation::Allele->new;
$a2->seq('t');
$rnachange->add_Allele($a2);
$rnachange->allele_mut($a2);
print "The codon change is ", $rnachange->codon_ori,
">", $rnachange->codon_mut, "\n";
# add it to a SeqDiff container object
$seqdiff->add_Variant($rnachange);
# and create links to and from DNA level mutation objects
$rnachange->DNAMutation($dnamut);
$dnamut->RNAChange($rnachange);

DESCRIPTION

The instantiable class Bio::Variation::DNAMutation describes basic sequence changes at RNA molecule level. It uses methods defined in superclass Bio::Variation::VariantI. See Bio::Variation::VariantI for details.

You are normally expected to create a corresponding Bio::Variation::DNAMutation object even if mutation is defined at RNA level. The numbering follows then cDNA numbering. Link the DNAMutation object to the RNAChange object using the method DNAMutation(). If the variation described by a RNAChange object is translated, link the corresponding Bio::Variation::AAChange object to it using method AAChange(). See Bio::Variation::DNAMutation and Bio::Variation::AAChange for more information.

FEEDBACK

Mailing Lists

User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing lists Your participation is much appreciated.

  [email protected]                  - General discussion
  http://bioperl.org/wiki/Mailing_lists  - About the mailing lists

Support

Please direct usage questions or support issues to the mailing list:

[email protected]

rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible.

Reporting Bugs

Report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via the web:

  https://github.com/bioperl/bioperl-live/issues

AUTHOR - Heikki Lehvaslaiho

Email: heikki-at-bioperl-dot-org

APPENDIX

The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _

codon_ori

 Title   : codon_ori
 Usage   : $obj->codon_ori();
 Function: 
            Sets and returns codon_ori triplet.  If value is not set,
            creates the codon triplet from the codon position and
            flanking sequences.  The string has to be three characters
            long. The character content is not checked.
 Example : 
 Returns : string
 Args    : string

codon_mut

 Title   : codon_mut
 Usage   : $obj->codon_mut();
 Function: 
            Sets and returns codon_mut triplet.  If value is not
            set, creates the codon triplet from the codon position and
            flanking sequences. Return undef for other than point mutations.
 Example : 
 Returns : string
 Args    : string

codon_pos

 Title   : codon_pos
 Usage   : $obj->codon_pos();
 Function: 
            Sets and returns the position of the mutation start in the
            codon. If value is not set, returns false.
 Example : 
 Returns : 1,2,3
 Args    : none if get, the new value if set

codon_table

 Title   : codon_table
 Usage   : $obj->codon_table();
 Function: 
            Sets and returns the codon table id of the RNA
            If value is not set, returns 1, 'universal' code, as the default.
 Example : 
 Returns : integer
 Args    : none if get, the new value if set

DNAMutation

 Title   : DNAMutation
 Usage   : $mutobj = $obj->DNAMutation;
         : $mutobj = $obj->DNAMutation($objref);
 Function: Returns or sets the link-reference to a mutation/change object.
           If there is no link, it will return undef
 Returns : an obj_ref or undef

AAChange

 Title   : AAChange
 Usage   : $mutobj = $obj->AAChange;
         : $mutobj = $obj->AAChange($objref);
 Function: Returns or sets the link-reference to a mutation/change object.
           If there is no link, it will return undef
 Returns : an obj_ref or undef

exons_modified

 Title   : exons_modified
 Usage   : $modified = $obj->exons_modified;
         : $modified = $obj->exons_modified(1);
 Function: Returns or sets information (example: a simple boolean flag) about
           the modification of exons as a result of a mutation.

region

 Title   : region
 Usage   : $obj->region();
 Function: 
            Sets and returns the name of the sequence region type or
            protein domain at this location.  If value is not set,
            returns false.
 Example : 
 Returns : string
 Args    : string

cds_end

 Title   : cds_end
 Usage   : $cds_end = $obj->get_cds_end();
 Function: 
           Sets or returns the cds_end from the beginning of the DNA sequence
           to the coordinate start used to describe variants.
           Should be the location of the last nucleotide of the
           terminator codon of the gene.
 Example : 
 Returns : value of cds_end, a scalar
 Args    :

label

 Title   : label
 Usage   : $obj->label();
 Function: 
            Sets and returns mutation event label(s).  If value is not
            set, or no argument is given returns false.  Each
            instantiable subclass of L<Bio::Variation::VariantI> needs
            to implement this method. Valid values are listed in
            'Mutation event controlled vocabulary' in
            http://www.ebi.ac.uk/mutations/recommendations/mutevent.html.
 Example : 
 Returns : string
 Args    : string

_change_codon_pos

 Title   : _change_codon_pos
 Usage   : $newCodonPos = _change_codon_pos($myCodonPos, 5)
 Function: 
           Keeps track of the codon position in a changeing sequence
 Returns : codon_pos = integer 1, 2 or 3
 Args    : valid codon position 
           signed integer offset to a new location in sequence