diffseq(1) Compare and report features of two similar sequences


diffseq -asequence sequence -bsequence sequence -wordsize integer [-globaldifferences boolean] -outfile report -aoutfeat featout -boutfeat featout
diffseq -help



is a command line program from EMBOSS ("the European Molecular Biology Open Software Suite"). It is part of the "Alignment:Differences" command group(s).


Input section

-asequence sequence

-bsequence sequence

Required section

-wordsize integer

The similar regions between the two sequences are found by creating a hash table of 'wordsize'd subsequences. 10 is a reasonable default. Making this value larger (20?) may speed up the program slightly, but will mean that any two differences within 'wordsize' of each other will be grouped as a single region of difference. This value may be made smaller (4?) to improve the resolution of nearby differences, but the program will go much slower. Default value: 10

Additional section

-globaldifferences boolean

Normally this program will find regions of identity that are the length of the specified word-size or greater and will then report the regions of difference between these matching regions. This works well and is what most people want if they are working with long overlapping nucleic acid sequences. You are usually not interested in the non-overlapping ends of these sequences. If you have protein sequences or short RNA sequences however, you will be interested in differences at the very ends . It this option is set to be true then the differences at the ends will also be reported. Default value: N

Output section

-outfile report

-aoutfeat featout

File for output of first sequence's features Default value: $(asequence.name).diffgff

-boutfeat featout

File for output of second sequence's features Default value: $(bsequence.name).diffgff


Bugs can be reported to the Debian Bug Tracking system (http://bugs.debian.org/emboss), or directly to the EMBOSS developers (http://sourceforge.net/tracker/?group_id=93650&atid=605031).


Debian Med Packaging Team <[email protected]>

Wrote the script used to autogenerate this manual page.


This manual page was autogenerated from an Ajax Control Definition of the EMBOSS package. It can be redistributed under the same terms as EMBOSS itself.