flanks --position POS [-p POS ...] [--flanklen INT]
accession | filename
DESCRIPTIONThis script allows you to extract a subsequence around a region of interest from an existing sequence. The output if fasta formatted sequence entry where the header line contains additional information about the location.
OPTIONSThe script takes one unnamed argument which be either a file name in the local file system or a nucleotide sequence accession number.
-p Position uses simple nucleotide sequence feature table --position notation to define the region of interest, typically a SNP or microsatellite repeat around which the flanks are defined. There can be more than one position option or you can give a comma separated list to one position option. The format of a position is: [id:] int | range | in-between [-] The optional id is the name you want to call the new sequence. If it not given in joins running number to the entry name with an underscore. The position is either a point (e.g. 234), a range (e.g 250..300) or insertion point between nucleotides (e.g. 234^235) If the position is not completely within the source sequence the output sequence will be truncated and it will print a warning. The optional hyphen [-] at the end of the position indicates that that you want the retrieved sequence to be in the opposite strand. -f Defaults to 100. This is the length of the nucleotides --flanklen sequence retrieved on both sides of the given position. If the source file does not contain
OUTPUT FORMATThe output is a fasta formatted entry where the description file contains tag=value pairs for information about where in the original sequence the subsequence was taken.
The ID of the fasta entry is the name given at the command line joined by hyphen to the filename or accesion of the source sequence. If no id is given a series of consequtive integers is used.
The tag=value pairs are:
- position in the source file
- strand of the sequence compared to the source sequence
- position of the region of interest in the current entry. The tag is the same as used by dbSNP@NCBI
The sequence highlights the allele variant position by showing it in upper case and rest of the sequence in lower case characters.
% flanks ~/seq/ar.embl >1_/HOME/HEIKKI/SEQ/AR.EMBL oripos=100 strand=1 allelepos=100 taataactcagttcttatttgcacctacttcagtggacactgaatttggaaggtggagga ttttgtttttttcttttaagatctgggcatcttttgaatCtacccttcaagtattaagag acagactgtgagcctagcagggcagatcttgtccaccgtgtgtcttcttctgcacgagac tttgaggctgtcagagcgct
TODOThe input files are assumed to be in EMBL format and the sequences are retrieved only from the EMB database. Make this more generic and use the registry.
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AUTHOR - Heikki LehvaslaihoEmail: <heikki-at-bioperl-dot-org>