SYNOPSIS
gt cds [option ...] [GFF3_file]
DESCRIPTION
-minorflen [value]
- set the minimum length an open reading frame (ORF) must have to be added as a CDS feature (measured in amino acids) (default: 64)
-startcodon [yes|no]
- require than an ORF must begin with a start codon (default: no)
-finalstopcodon [yes|no]
- require that the final ORF must end with a stop codon (default: no)
-seqfile [filename]
- set the sequence file from which to take the sequences (default: undefined)
-encseq [filename]
- set the encoded sequence indexname from which to take the sequences (default: undefined)
-seqfiles
- set the sequence files from which to extract the features use -- to terminate the list of sequence files
-matchdesc [yes|no]
- search the sequence descriptions from the input files for the desired sequence IDs (in GFF3), reporting the first match (default: no)
-matchdescstart [yes|no]
- exactly match the sequence descriptions from the input files for the desired sequence IDs (in GFF3) from the beginning to the first whitespace (default: no)
-usedesc [yes|no]
- use sequence descriptions to map the sequence IDs (in GFF3) to actual sequence entries. If a description contains a sequence range (e.g., III:1000001..2000000), the first part is used as sequence ID (III) and the first range position as offset (1000001) (default: no)
-regionmapping [string]
- set file containing sequence-region to sequence file mapping (default: undefined)
-v [yes|no]
- be verbose (default: no)
-o [filename]
- redirect output to specified file (default: undefined)
-gzip [yes|no]
- write gzip compressed output file (default: no)
-bzip2 [yes|no]
- write bzip2 compressed output file (default: no)
-force [yes|no]
- force writing to output file (default: no)
-help
- display help and exit
-version
- display version information and exit
File format for option -regionmapping:
The file supplied to option -regionmapping defines a "mapping". A mapping maps the sequence-region entries given in the GFF3_file to a sequence file containing the corresponding sequence. Mappings can be defined in one of the following two forms:
-
mapping = { chr1 = "hs_ref_chr1.fa.gz", chr2 = "hs_ref_chr2.fa.gz" }
or
-
function mapping(sequence_region) return "hs_ref_"..sequence_region..".fa.gz" end
The first form defines a Lua (http://www.lua.org) table named "mapping" which maps each sequence region to the corresponding sequence file. The second one defines a Lua function "mapping", which has to return the sequence file name when it is called with the sequence_region as argument.