SYNOPSIS
mauveAligner [options] <seq1 filename> <sml1 filename> ... <seqN filename> <smlN filename>DESCRIPTION
The mauveAligner and progressiveMauve alignment algorithms have been implemented as command-line programs included with the downloadable Mauve software. When run from the command-line, these programs provide options not yet available in the graphical interface.OPTIONS
- --output=<file> Output file name.
- Prints to screen by default
- --mums Find MUMs only, do not attempt to determine locally collinear blocks (LCBs)
- --no-recursion Don't perform recursive anchor identification (implies --no-gapped-alignment)
- --no-lcb-extension If determining LCBs, don't attempt to extend the LCBs
- --seed-size=<number> Initial seed match size, default is log_2( average seq. length )
- --max-extension-iterations=<number> Limit LCB extensions to this number of attempts, default is 4
- --eliminate-inclusions Eliminate linked inclusions in subset matches.
- --weight=<number> Minimum LCB weight in base pairs per sequence
- --match-input=<file> Use specified match file instead of searching for matches
- --lcb-match-input Indicates that the match input file contains matches that have been clustered into LCBs
- --lcb-input=<file> Use specified lcb file instead of constructing LCBs (skips LCB generation)
- --scratch-path=<path> For large genomes, use a directory for storage of temporary data. Should be given two or more times to with different paths.
- --id-matrix=<file> Generate LCB stats and write them to the specified file
- --island-size=<number> Find islands larger than the given number
- --island-output=<file> Output islands the given file (requires --island-size)
- --backbone-size=<number> Find stretches of backbone longer than the given number of b.p.
- --max-backbone-gap=<number> Allow backbone to be interrupted by gaps up to this length in b.p.
- --backbone-output=<file> Output islands the given file (requires --island-size)
- --coverage-output=<file> Output a coverage list to the specified file (- for stdout)
- --repeats Generates a repeat map. Only one sequence can be specified
- --output-guide-tree=<file> Write out a guide tree to the designated file
- --collinear Assume that input sequences are collinear--they have no rearrangements
Gapped alignment controls:
- --no-gapped-alignment Don't perform a gapped alignment
- --max-gapped-aligner-length=<number> Maximum number of base pairs to attempt aligning with the gapped aligner
- --min-recursive-gap-length=<number> Minimum size of gaps that Mauve will perform recursive MUM anchoring on (Default is 200)
Signed permutation matrix options:
- --permutation-matrix-output=<file> Write out the LCBs as a signed permutation matrix to the given file
- --permutation-matrix-min-weight=<number> A permutation matrix will be written for every set of LCBs with weight between this value and the value of --weight
Alignment output options:
- --alignment-output-dir=<directory> Outputs a set of alignment files (one per LCB) to a given directory
- --alignment-output-format=<directory> Selects the output format for --alignment-output-dir
- --output-alignment=<file> Write out an XMFA format alignment to the designated file
Supported alignment output formats are: phylip, clustal, msf, nexus, mega, codon