SYNOPSIS:

phyml [command args]

All the options below are optional (except '-i' if you want to use the command-line interface).

Command options:

-i (or --input) seq_file_name

seq_file_name is the name of the nucleotide or amino-acid sequence file in PHYLIP format.

-d (or --datatype) data_type

data_type is 'nt' for nucleotide (default), 'aa' for amino-acid sequences, or 'generic', (use NEXUS file format and the 'symbols' parameter here).

-q (or --sequential)

Changes interleaved format (default) to sequential format.

-n (or --multiple) nb_data_sets

nb_data_sets is an integer corresponding to the number of data sets to analyse.

-p (or --pars) [

Use a minimum parsimony starting tree. This option is taken into account when the '-u' option is absent and when tree topoLOGy modifications are to be done.

-b (or --bootstrap) int

int > 0: int is the number of bootstrap replicates.

int = 0: neither approximate likelihood ratio test nor bootstrap values are computed.

int = -1: approximate likelihood ratio test returning aLRT statistics.

int = -2: approximate likelihood ratio test returning Chi2-based parametric branch supports.

int = -4: (default) SH-like branch supports alone.

-m (or --model) model

model : substitution model name. - Nucleotide-based models : HKY85 (default) | JC69 | K80 | F81 | F84 | TN93 | GTR | custom (for the custom option, a string of six digits identifies the model. For instance, 000000)

corresponds to F81 (or JC69 provided the distribution of nucleotide frequencies is uniform). 012345 corresponds to GTR. This option can be used for encoding any model that is a nested within GTR.

- Amino-acid based models : LG (default) | WAG | JTT | MtREV | Dayhoff | DCMut | RtREV | CpREV | VT Blosum62 | MtMam | MtArt | HIVw | HIVb | custom

--aa_rate_file filename

filename is the name of the file that provides the amino acid substitution rate matrix in PAML format.

It is compulsory to use this option when analysing amino acid sequences with the `custom' model.

-f e, m, or fA,fC,fG,fT

e : the character frequencies are determined as follows :

- Nucleotide sequences: (Empirical) the equilibrium base frequencies are estimated by counting

the occurence of the different bases in the alignment.

- Amino-acid sequences: (Empirical) the equilibrium amino-acid frequencies are estimated by counting the occurence of the different amino-acids in the alignment.

m : the character frequencies are determined as follows :

- Nucleotide sequences: (ML) the equilibrium base frequencies are estimated using maximum likelihood

- Amino-acid sequences: (Model) the equilibrium amino-acid frequencies are estimated using the frequencies defined by the substitution model.

"fA,fC,fG,fT" : only valid for nucleotide-based models. fA, fC, fG and fT are floating numbers that correspond to the frequencies of A, C, G and T respectively (WARNING: do not use any blank space between your values of nucleotide frequencies, only commas!)

-t (or --ts/tv) ts/tv_ratio

ts/tv_ratio : transition/transversion ratio. DNA sequences only.

Can be a fixed positive value (ex:4.0) or e to get the maximum likelihood estimate.

-v (or --pinv) prop_invar

prop_invar: proportion of invariable sites.

Can be a fixed value in the [0,1] range or e to get the maximum likelihood estimate.

-c (or --nclasses) nb_subst_cat

nb_subst_cat : number of relative substitution rate categories. Default: nb_subst_cat=4.

Must be a positive integer.

-a (or --alpha) gamma

gamma : distribution of the gamma distribution shape parameter.

Can be a fixed positive value or e to get the maximum likelihood estimate.

-s (or --search) move

Tree topoLOGy search operation option. Can be either NNI (default, fast) or SPR (a bit slower than NNI) or BEST (best of NNI and SPR search).

-u (or --inputtree) user_tree_file

user_tree_file : starting tree filename. The tree must be in Newick format.

-o params

This option focuses on specific parameter optimisation.

params=tlr : tree topoLOGy (t), branch length (l) and rate parameters (r) are optimised.

params=tl : tree topoLOGy and branch length are optimised.

params=lr : branch length and rate parameters are optimised.

params=l : branch length are optimised.

params=r : rate parameters are optimised.

params=n : no parameter is optimised.

--rand_start

This option sets the initial tree to random. It is only valid if SPR searches are to be performed.

--n_rand_starts num

num is the number of initial random trees to be used.

It is only valid if SPR searches are to be performed.

--r_seed num

num is the seed used to initiate the random number generator.

Must be an integer.

--print_site_lnl

Print the likelihood for each site in file *_phyml_lk.txt.

--print_trace

Print each phyLOGeny explored during the tree search process in file *_phyml_trace.txt.

--run_id ID_string

Append the string ID_string at the end of each PhyML output file. This option may be useful when running simulations involving PhyML.

--quiet

No interactive question (for running in batch mode) and quiet output.

--no_memory_check

No interactive question for memory usage (for running in batch mode). Normal output otherwise.

--alias_subpatt

Site aliasing is generalized at the subtree level. Sometimes lead to faster calculations. See Kosakovsky Pond SL, Muse SV, Sytematic Biology (2004) for an example.

--boot_progress_display num (default=20)

num is the frequency at which the bootstrap progress bar will be updated.

Must be an integer.

PHYLIP-LIKE INTERFACE

You can also use PhyML with no argument, in this case change the value of a parameter by typing its corresponding character as shown on screen.

EXAMPLES

DNA interleaved sequence file, default parameters :

phyml -i seqs1

AA interleaved sequence file, default parameters :

phyml -i seqs2 -d aa

AA sequential sequence file, with customization :

phyml -i seqs3 -q -d aa -m JTT -c 4 -a e

AUTHOR

PhyML was written by Stephane Guindon and Olivier Gascuel and others

This manual page was written by Andreas Tille <[email protected]>, for the Debian project (but may be used by others).