PyNAST(1)
alignment of short DNA sequences
SYNOPSIS
pynast
[options] {-i input_fp -t template_fp}
DESCRIPTION
[] indicates optional input (order unimportant)
{} indicates required input (order unimportant)
Example usage:
-
pynast -i my_input.fasta -t my_template.fasta
OPTIONS
- --version
-
show program's version number and exit
- -h, --help
-
show this help message and exit
- -t TEMPLATE_FP, --template_fp=TEMPLATE_FP
-
path to template alignment file [REQUIRED]
- -i INPUT_FP, --input_fp=INPUT_FP
-
path to input fasta file [REQUIRED]
- -v, --verbose
-
Print status and other information during execution
[default: False]
- -p MIN_PCT_ID, --min_pct_id=MIN_PCT_ID
-
minimum percent sequence identity to consider a
sequence a match [default: 75.0]
- -l MIN_LEN, --min_len=MIN_LEN
-
minimum sequence length to include in NAST alignment
[default: 1000]
- -m PAIRWISE_ALIGNMENT_METHOD, --pairwise_alignment_method=PAIRWISE_ALIGNMENT_METHOD
-
method for performing pairwise alignment [default:
uclust]
- -a FASTA_OUT_FP, --fasta_out_fp=FASTA_OUT_FP
-
path to store resulting alignment file [default:
derived from input filepath]
- -g LOG_FP, --log_fp=LOG_FP
-
path to store log file [default: derived from input
filepath]
- -f FAILURE_FP, --failure_fp=FAILURE_FP
-
path to store file of seqs which fail to align
[default: derived from input filepath]
- -e MAX_E_VALUE, --max_e_value=MAX_E_VALUE
-
Depreciated. Will be removed in PyNAST 1.2
- -d BLAST_DB, --blast_db=BLAST_DB
-
Depreciated. Will be removed in PyNAST 1.2