USAGE
qualityScores [options] -i <input_file> -o <output_file>
Required arguments:
- -i <string>
- Name of input file including read data. The default format is Fastq.
- -o <string>
- Name of output file that is a text file including Phred scores for each read base.
Optional arguments:
-
--gzFASTQinput Input file is in gzipped Fastq format.
- --BAMinput
-
- Input file is in BAM format.
- --SAMinput
- Input file is in SAM format.
- --first-end
- Use only first reads in paired-end data. Only applicable for paired-end BAM/SAM input.
- --second-end
- Use only second reads in paired-end data. Only applicable for paired-end BAM/SAM input.
- --counted-reads <int> Total number of reads to be extracted from the input
-
- file. 10,000 by default.
-
--phred-offset <33|64> refer to subread aligner.