wigeon(1) reimplementation of the Pintail 16S DNA anomaly detection utility


WigeoN examines the sequence conservation between a query and a trusted reference sequence, both in NAST alignment format. Based on the sequence identity between the query and the reference sequence, there is an expected amount of variation among the alignment. If the observed variation is greater than the 95% quantile of the distribution of variation observed between non-anomalous sequences, then it is flagged as an anomaly.

WigeoN is a flexible command-line based reimplementation of the Pintail algorithm Appl Environ Microbiol. 2005 Dec;7112:7724-36.

WigeoN is useful for flagging chimeras and anomalies only in near full-length 16S rRNA sequences. WigeoN lacks sensitivity with sequences less than 1000 bp.

To run WigeoN, you need NAST-formatted sequences generated by the nast-ier utility.

WigeoN is part of the microbiomeutil suite.



multi-fasta file containing query sequences in alignment format


db in NAST format
db in fasta format (megablast formatted)
default 1: uses only the single best match.
cd to exec_dir before running


This manual page was written by Andreas Tille <[email protected]> but can be freely used for any other distribution.