Bio::Restriction::Enzyme::MultiCut(3) A single restriction endonuclease

SYNOPSIS


# set up a single restriction enzyme. This contains lots of
# information about the enzyme that is generally parsed from a
# rebase file and can then be read back
use Bio::Restriction::Enzyme;

DESCRIPTION

This module defines a restriction endonuclease class where one object represents one of the distinct recognition sites for that enzyme. The method others stores references to other objects with alternative sites.

In this schema each object within an EnzymeCollection can be checked for matching a sequence.

REBASE report notation "Bsp24I (8/13)GACNNNNNNTGG(12/7)" means: 

   Bsp24I
             5'      ^NNNNNNNNGACNNNNNNTGGNNNNNNNNNNNN^   3'
             3' ^NNNNNNNNNNNNNCTGNNNNNNACCNNNNNNN^        5'

FEEDBACK

Mailing Lists

User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to one of the Bioperl mailing lists. Your participation is much appreciated. 

  [email protected]                  - General discussion
  http://bioperl.org/wiki/Mailing_lists  - About the mailing lists

Support

Please direct usage questions or support issues to the mailing list:

[email protected]

rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible.

Reporting Bugs

Report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via the web: 

  https://github.com/bioperl/bioperl-live/issues

AUTHOR

Heikki Lehvaslaiho, heikki-at-bioperl-dot-org

CONTRIBUTORS

Rob Edwards, [email protected]

COPYRIGHT

Copyright (c) 2003 Rob Edwards.

Some of this work is Copyright (c) 1997-2002 Steve A. Chervitz. All Rights Reserved. This module is free software; you can redistribute it and/or modify it under the same terms as Perl itself.

APPENDIX

Methods beginning with a leading underscore are considered private and are intended for internal use by this module. They are not considered part of the public interface and are described here for documentation purposes only.

new

 Title     : new
 Function
 Function  : Initializes the enzyme object
 Returns   : The Restriction::Enzyme::MultiCut object
 Argument  :

others

 Title     : others
 Usage     : $re->others(@enz_obj_array);
 Function  : Stores auxiliary Enzyme::MultiCut objects for multicutting enzymes
 Arguments : optional array of Enzyme::MultiCut objects
 Returns   : array of Enzyme objects

Added for compatibility to REBASE

purge_others

 Title     : purge_others
 Usage     : $re->purge_references();
 Function  : Purges the set of references for this enzyme
 Arguments : 
 Returns   :

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