SYNOPSIS
# Working with iterations (PSI-BLAST results)
$result->next_iteration();
$result->num_iterations();
$result->iteration();
$result->iterations();
# See Bio::Search::Result::GenericResult for information about working with Results.
# See Bio::Search::Iteration::IterationI # for details about working with iterations.
# TODO: # * Show how to configure a SearchIO stream so that it generates # BlastResult objects.
DESCRIPTION
This object is a subclass of Bio::Search::Result::GenericResult and provides some operations that facilitate working with BLAST and PSI-BLAST results.For general information about working with Results, see Bio::Search::Result::GenericResult.
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AUTHOR - Steve Chervitz
Email [email protected]APPENDIX
The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _new
Title : new
Usage : my $obj = Bio::Search::Result::BlastResult->new();
Function: Builds a new Bio::Search::Result::BlastResult object
Returns : Bio::Search::Result::BlastResult
Args : See Bio::Search::Result::GenericResult();
The following parameters are specific to BlastResult:
-iterations => array ref of Bio::Search::Iteration::IterationI objects
-inclusion_threshold => e-value threshold for inclusion in the
PSI-BLAST score matrix model (blastpgp)
hits
Title : hits
Usage : my @hits = $result->hits
Function: Returns the available hits for this Result
Returns : Array of L<Bio::Search::Hit::HitI> objects
Args : none
Note : This method overrides L<Bio::Search::Result::GenericResult::hits> to
take into account the possibility of multiple iterations, as occurs
in PSI-BLAST reports.
If there are multiple iterations, all 'new' hits for all iterations
are returned. These are the hits that did not occur in a previous
iteration.
See Also: L<Bio::Search::Result::GenericResult::hits>
next_hit
Title : next_hit
Usage : while( $hit = $result->next_hit()) { ... }
Function: Returns the next available Hit object, representing potential
matches between the query and various entities from the database.
Returns : a Bio::Search::Hit::HitI object or undef if there are no more.
Args : none
Note : This method overrides L<Bio::Search::Result::GenericResult::next_hit>
to take into account the possibility of multiple iterations, as
occurs in PSI-BLAST reports.
If there are multiple iterations, calling next_hit() traverses the
all of the hits, old and new, for each iteration, calling next_hit()
on each iteration.
See Also: L<Bio::Search::Iteration::GenericIteration::next_hit>
num_hits
Title : num_hits
Usage : my $hitcount= $result->num_hits
Function: returns the number of hits for this query result
Returns : integer
Args : none
Note : This method overrides L<Bio::Search::Result::GenericResult::num_hits>
to take into account the possibility of multiple iterations, as
occurs in PSI-BLAST reports.
If there are multiple iterations, calling num_hits() returns the
number of 'new' hits for each iteration. These are the hits that did
not occur in a previous iteration.
See Also: L<Bio::Search::Result::GenericResult::num_hits>
add_hit
Title : add_hit Usage : $report->add_hit($hit) Function: Adds a HitI to the stored list of hits Returns : Number of HitI currently stored Args : Bio::Search::Hit::HitI
add_iteration
Title : add_iteration Usage : $report->add_iteration($iteration) Function: Adds a IterationI to the stored list of iterations Returns : Number of IterationI currently stored Args : Bio::Search::Iteration::IterationI
next_iteration
Title : next_iteration
Usage : while( $it = $result->next_iteration()) { ... }
Function: Returns the next Iteration object, representing all hits
found within a given PSI-Blast iteration.
Returns : a Bio::Search::Iteration::IterationI object or undef if there are no more.
Args : none
iteration
Usage : $iteration = $blast->iteration( $number );
Purpose : Get an IterationI object for the specified iteration
in the search result (PSI-BLAST).
Returns : Bio::Search::Iteration::IterationI object
Throws : Bio::Root::NoSuchThing exception if $number is not within
range of the number of iterations in this report.
Argument : integer (optional, if not specified get the last iteration)
First iteration = 1
num_iterations
Usage : $num_iterations = $blast->num_iterations; Purpose : Get the number of iterations in the search result (PSI-BLAST). Returns : Total number of iterations in the report Argument : none (read-only)
number_of_iterations
Usage : $num_iterations = $blast->number_of_iterations; Purpose : Get the number of iterations in the search result (PSI-BLAST). Returns : Total number of iterations in the report Argument : none (read-only) Note : Alias of L<num_iterations>.
round
Usage : $round = $blast->round( $number );
Purpose : Get an IterationI object for the specified iteration
in the search result (PSI-BLAST).
Returns : Bio::Search::Iteration::IterationI object
Throws : Bio::Root::NoSuchThing exception if $number is not within
range of the number of iterations in this report.
Argument : integer (optional, if not specified get the last iteration)
First iteration = 1
Note : Alias of L<iteration>.
iterations
Title : iterations Usage : my @iterations = $result->iterations Function: Returns the IterationI objects contained within this Result Returns : Array of L<Bio::Search::Iteration::IterationI> objects Args : none
psiblast
Usage : if( $blast->psiblast ) { ... }
Purpose : Set/get a boolean indicator whether or not the report
is a PSI-BLAST report.
Returns : 1 if PSI-BLAST, undef if not.
Argument : 1 (when setting)
no_hits_found
Usage : $nohits = $blast->no_hits_found( $iteration_number );
Purpose : Get boolean indicator indicating whether or not any hits
were present in the report.
This is NOT the same as determining the number of hits via
the hits() method, which will return zero hits if there were no
hits in the report or if all hits were filtered out during the parse.
Thus, this method can be used to distinguish these possibilities
for hitless reports generated when filtering.
Returns : Boolean
Argument : (optional) integer indicating the iteration number (PSI-BLAST)
If iteration number is not specified and this is a PSI-BLAST result,
then this method will return true only if all iterations had
no hits found.
set_no_hits_found
Usage : $blast->set_no_hits_found( $iteration_number );
Purpose : Set boolean indicator indicating whether or not any hits
were present in the report.
Returns : n/a
Argument : (optional) integer indicating the iteration number (PSI-BLAST)
_next_iteration_index
Title : _next_iteration_index Usage : private
rewind
Title : rewind
Usage : $result->rewind;
Function: Allow one to reset the Iteration iterator to the beginning
Since this is an in-memory implementation
Returns : none
Args : none
inclusion_threshold
Title : inclusion_threshold
Usage : my $incl_thresh = $result->inclusion_threshold; (read-only)
Function: Gets the e-value threshold for inclusion in the PSI-BLAST
score matrix model (blastpgp) that was used for generating the report
being parsed.
Returns : number (real) or undef if not a PSI-BLAST report.
Args : none