SYNOPSIS

use Bio::Tools::Run::Alignment::Lagan;
@params =
('chaos' => "The contents of this string will be passed as args to chaos",
#Read you chaos README file for more info/This functionality
#has not been tested and will be integrated in future versions.
'order' => "\"-gs -7 -gc -2 -mt 2 -ms -1\"",
#Where gap start penalty of- 7, gap continue of -2, match of 2,
#and mismatch of -1.
'recurse' => "\"(12,25),(7,25),(4,30)"\",
#A list of (wordlength,score cutoff) pairs to be used in the
#recursive anchoring
'tree' => "\"(sample1 (sample2 sample3))"\",
#Used by mlagan / tree can also be passed when calling mlagan directly
#SCORING PARAMETERS FOR MLAGAN:
'match' => 12,
'mismatch' => -8,
'gapstart' => -50,
'gapend' => -50,
'gapcont' => -2,
);

DESCRIPTION

To run mlagan/lagan, you must have an environment variable that points to the executable directory with files lagan.pl etc. ``LAGAN_DIR=/opt/lagan_executables/''

Simply having the executables in your path is not supported because the executables themselves only work with the environment variable set.

All lagan and mlagan parameters listed in their Readmes can be set except for the mfa flag which has been turned on by default to prevent parsing of the alignment format.

TO USE LAGAN:

  my $lagan = Bio::Tools::Run::Alignment::Lagan->new(@params);
  my $report_out = $lagan->lagan($seq1, $seq2);

A SimpleAlign object is returned.

TO USE MLAGAN:

  my $lagan = Bio::Tools::Run::Alignment::Lagan->new();
  my $tree = "(($seqname1 $seqname2) $seqname3)";
  my @sequence_objs;    #an array of bioperl Seq objects
  ##If you use an unblessed seq array
  my $seq_ref = \@sequence_objs;
  bless $seq_ref, "ARRAY";
  my $report_out = $lagan->mlagan($seq_ref, $tree);
  A SimpleAlign object is returned

Only basic mlagan/lagan functionality has been implemented due to the iterative development of their project. Future maintenance upgrades will include enhanced features and scoring.

FEEDBACK

Mailing Lists

User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing list. Your participation is much appreciated.

  [email protected]                  - General discussion
  http://bioperl.org/wiki/Mailing_lists  - About the mailing lists

Support

Please direct usage questions or support issues to the mailing list:

[email protected]

rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible.

Reporting Bugs

Report bugs to the Bioperl bug tracking system to help us keep track of the bugs and their resolution. Bug reports can be submitted via the web:

  http://redmine.open-bio.org/projects/bioperl/

AUTHOR - Stephen Montgomery

Email [email protected]

Genome Sciences Centre in beautiful Vancouver, British Columbia CANADA

CONTRIBUTORS

MLagan/Lagan is the hard work of Michael Brudno et al.

Sendu Bala [email protected]

APPENDIX

The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _

lagan

  Runs the Lagan pairwise alignment algorithm
  Inputs should be two PrimarySeq objects.
  Returns an SimpleAlign object / preloaded with the tmp file of the
  Lagan multifasta output.

mlagan

  Runs the Mlagan multiple sequence alignment algorithm.
  Inputs should be an Array of Primary Seq objects and a Phylogenetic Tree in
  String format or as a Bio::Tree::TreeI compliant object.
  Returns an SimpleAlign object / preloaded with the tmp file of the Mlagan
  multifasta output.

nuc_matrix

 Title   : nuc_matrix
 Usage   : my $matrix_obj = $obj->nuc_matrix();
           -or-
           $obj->nuc_matrix($matrix_obj);
           -or-
           $obj->nuc_matrix($matrix_file);
 Function: Get/set the substitution matrix for use by mlagan. By default the
           file $LAGAN_DIR/nucmatrix.txt is used by mlagan. By default this
           method returns a corresponding Matrix.
 Returns : Bio::Matrix::Mlagan object
 Args    : none to get, OR to set:
           Bio::Matrix::MLagan object
           OR
           filename of an mlagan substitution matrix file
           NB: due to a bug in mlagan 2.0, the -nucmatrixfile option does not
           work, so this Bioperl wrapper is unable to simply point mlagan to
           your desired matrix file (or to a temp file generated from your
           matrix object). Instead the $LAGAN_DIR/nucmatrix.txt file must
           actually be replaced. This wrapper will make a back-up copy of that
           file, write the new file in its place, then revert things back to the
           way they were after the alignment has been produced. For this reason,
           $LAGAN_DIR must be writable, as must $LAGAN_DIR/nucmatrix.txt.

_setinput

 Title   : _setinput
 Usage   : Internal function, not to be called directly
 Function: Create input file(s) for Lagan executables
 Returns : name of files containing Lagan data input / 
           or array of files and phylo tree for Mlagan data input

_generic_lagan

 Title   : _generic_lagan
 Usage   :  internal function not called directly
 Returns :  SimpleAlign object

_setparams

 Title   : _setparams
 Usage   : Internal function, not to be called directly
 Function: Create parameter inputs for (m)Lagan program
 Returns : parameter string to be passed to Lagan
 Args    : Reference to calling object and name of (m)Lagan executable

_runlagan

 Title   :  _runlagan
 Usage   :  Internal function, not to be called directly
 Function:   makes actual system call to (m)Lagan program
 Example :
 Returns : Report object in the SimpleAlign object

executable

 Title   : executable
 Usage   : my $exe = $lagan->executable('mlagan');
 Function: Finds the full path to the 'lagan' executable
 Returns : string representing the full path to the exe
 Args    : [optional] name of executable to set path to
           [optional] boolean flag whether or not warn when exe is not found
 Thanks to Jason Stajich for providing the framework for this subroutine

program_path

 Title   : program_path
 Usage   : my $path = $lagan->program_path();
 Function: Builds path for executable
 Returns : string representing the full path to the exe
 Thanks to Jason Stajich for providing the framework for this subroutine

program_dir

 Title   : program_dir
 Usage   : my $dir = $lagan->program_dir();
 Function: Abstract get method for dir of program. To be implemented
           by wrapper.
 Returns : string representing program directory
 Thanks to Jason Stajich for providing the framework for this subroutine

version

 Title   : version
 Usage   : my $version = $lagan->version;
 Function: returns the program version
 Returns : number
 Args    : none