SYNOPSIS
homGeneMapping [options] --gtfs=gffilenames.tbl --halfile=aln.hal
DESCRIPTION
homGeneMapping takes a set of gene predictions of different genomes and a hal alignment of the genomes and prints a summary for each gene, e.g.
- • how many of its exons/introns are in agreement with genes of other genomes
- • how many of its exons/introns are supported by extrinsic evidence from any of the genomes
- • a list of geneids of homologous genes
OPTIONS
Mandatory parameters
--halfile=aln.hal
- input hal file
--gtfs=gtffilenames.tbl
-
a text file containing the locations of the input gene files
and optionally the hints files (both in GTF format).
The file is formatted as follows:
-
name_of_genome_1 path/to/genefile/of/genome_1 path/to/hintsfile/of/genome_1 name_of_genome_2 path/to/genefile/of/genome_2 path/to/hintsfile/of/genome_2 ... name_of_genome_N path/to/genefile/of/genome_N path/to/hintsfile/of/genome_N
-
Additional options
--cpus=N
- N is the number of CPUs to use (default: 1)
--noDupes
- do not map between duplications in hal graph. (default: off)
--halLiftover_exec_dir=DIR
- Directory that contains the executable halLiftover If not specified it must be in $PATH environment variable.
--tmpdir=DIR
- a temporary file directory that stores lifted over files. (default 'tmp/' in current directory)
--outdir=DIR
- file direcory that stores output gene files. (default: current directory)
--printHomologs=FILE
-
prints disjunct sets of homologous transcripts to FILE, e.g.
-
# 0 dana # 1 dere # 2 dgri # 3 dmel # 4 dmoj # 5 dper (0, jg4139.t1) (0, jg4140.t1) (1, jg7797.t1) (2, jg3247.t1) (4, jg6720.t1) (5, jg313.t1) (1, jg14269.t1) (3, jg89.t1) (5, jg290.t1) ... Two transcripts are in the same set, if all their exons/introns are homologs and their are no additional exons/introns. This option requires the Boost C++ Library
-
EXAMPLE
homGeneMapping --noDupes --halLiftover_exec_dir=~/tools/progressiveCactus/submodules/hal/bin --gtfs=gtffilenames.tbl --halfile=msca.hal
AUTHORS
AUGUSTUS was written by M. Stanke, O. Keller, S. König, L. Gerischer and L. Romoth.
ADDITIONAL DOCUMENTATION
An exhaustive documentation can be found in the file /usr/share/augustus/README.TXT.