command-line interface Bio::PrimerDesigner


./ [options] [dna_sequence or file]
-p|--program Program (default "primer3")
-n|--number Number of primer sets to return (default "5")
-b|--binary Path to binary executable (default "/usr/local/bin")
-m|--method "local" or "remote" (default "local")
-u|--url URL of remote primer3/e-PCR system (default
--list-aliases Print alias list to primer3 input options and exit
--list-params Print a list of primer3/e-PCR input options and exit
-h|--help Print help and exit


This script tests/demonstrates the Bio::PrimerDesigner interface to primer3 and e-PCR. It can be used with a local installation of the unix binaries or remotely via an HTTP/CGI request.


Copyright (C) 2003-2009 Sheldon McKay <[email protected]>, Ken Youens-Clark <[email protected]>.


This program is free software; you can redistribute it and/or modify it under the terms of the GNU General Public License as published by the Free Software Foundation; version 3 or any later version.

This program is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more details.

You should have received a copy of the GNU General Public License along with this program; if not, write to the Free Software Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301 USA.