SYNOPSIS

use Bio::Tools::Run::Match;
# Make a Match factory
$factory = Bio::Tools::Run::Match->new(-mxlib => '/path/to/matrix.dat');
# Run Match on an sequence object
my @results = $factory->run($bio_seq);
# look at the results
foreach my $feat (@results) {
my $seq_id = $feat->seq_id;
my $start = $feat->start;
my $end = $feat->end;
my $score = $feat->score;
my ($pvalue) = $feat->get_tag_values('pvalue');
}

DESCRIPTION

This is a wrapper for running the match(TM) program supplied with Transfac Pro distributions.

You can try supplying normal match command-line arguments to new(), eg. new(-b => 1) or calling arg-named methods (excluding the initial hyphens, eg. $factory->b(1) to set the -b option to true).

Histogram output isn't supported. -p is supported by using -mxprf, see the docs of new() for details.

You will need to enable this match wrapper to find the match executable. This can be done in (at least) three ways:

 1. Make sure match is in your path.
 2. Define an environmental variable MATCHDIR which is a 
    directory which contains the match executable:
    In bash:
    export MATCHDIR=/home/username/match/
    In csh/tcsh:
    setenv MATCHDIR /home/username/match
 3. Include a definition of an environmental variable MATCHDIR in
    every script that will use this match wrapper module, e.g.:
    BEGIN { $ENV{MATCHDIR} = '/home/username/match/' }
    use Bio::Tools::Run::Match;

FEEDBACK

Mailing Lists

User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing list. Your participation is much appreciated.

  [email protected]                  - General discussion
  http://bioperl.org/wiki/Mailing_lists  - About the mailing lists

Support

Please direct usage questions or support issues to the mailing list:

[email protected]

rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible.

Reporting Bugs

Report bugs to the Bioperl bug tracking system to help us keep track of the bugs and their resolution. Bug reports can be submitted via the web:

  http://redmine.open-bio.org/projects/bioperl/

AUTHOR - Sendu Bala

Email [email protected]

APPENDIX

The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _

program_name

 Title   : program_name
 Usage   : $factory>program_name()
 Function: holds the program name
 Returns : string
 Args    : None

program_dir

 Title   : program_dir
 Usage   : $factory->program_dir(@params)
 Function: returns the program directory, obtained from ENV variable.
 Returns : string
 Args    : None

new

 Title   : new
 Usage   : $factory = Bio::Tools::Run::Match->new()
 Function: creates a new MCS factory
 Returns : Bio::Tools::Run::MCS
 Args    : The following args can either be supplied here or set by calling
           arg-named methods (eg. $factory->imcut(2) ).
           -mxlib  => path to the matrix.dat file containing Transfac matricies
           -mxprf  => path to a profile file | [core_thresh, [matrix_thresh]]
                      (defaults to a standard one based on the mxlib provided if
                      file not supplied, using core_thresh and matrix_thresh
                      values if those are supplied instead)
           -imcut  => floating point number, the importance cutoff
           -b | -u => boolean, mutually exclusive

run

 Title   : run
 Usage   : $result = $factory->run($bio_seqi_object);
 Function: Runs match on a sequence.
 Returns : list of Bio::SeqFeatureI feature objects
 Args    : Bio::SeqI compliant object
           NB: mxlib has to have been set prior to calling run(), either as an
           argument to new() or by calling mxlib().

_setparams

 Title   : _setparams
 Usage   : Internal function, not to be called directly
 Function: Creates a string of params to be used in the command string
 Returns : string of params
 Args    : none