man probcons (1): align multiple protein sequences and print to standard output

SYNOPSIS

probcons [OPTION] [MFAFILE] [MFAFILE]

DESCRIPTION

probcons

is a tool for generating multiple alignments of protein sequences. Using a combination of probabilistic modeling and consistency-based alignment techniques, PROBCONS has achieved the highest accuracies of all alignment methods to date. On the BAliBASE benchmark alignment database, alignments produced by PROBCONS show statistically significant improvement over current programs, containing an average of 7% more correctly aligned columns than those of T-Coffee, 11% more correctly aligned columns than those of CLUSTAL W, and 14% more correctly aligned columns than those of DIALIGN.

probcons aligns sequences provided in MFA format. This format consists of multiple sequences. Each sequence in MFA format begins with a single-line description, followed by lines of sequence data. The description line is distinguished from the sequence data by a greater-than (">") symbol in the first column.

OPTIONS

-clustalw

: use CLUSTALW output format instead of MFA

-c --consistency REPS

: use 0 <= REPS <= 5 (default: 2) passes of consistency transformation

-ir --iterative-refinement REPS

: use 0 <= REPS <=1000 (default: 100) passes of iterative-refinement

-pre --pre-training REPS

: use 0 <= REPS <= 20 (default: 0) rounds of pretraining

-pairs

: generate all-pairs pairwise alignments

-viterbi

: use Viterbi algorithm to generate all pairs (automatically enables -pairs)

-v --verbose

: Report progress while aligning (default: off)

-annot FILENAME

: write annotation for multiple alignment to FILENAME

-t --train FILENAME

: compute EM transition probabilities, store in FILENAME (default: no training)

-e --emissions

: also reestimate emission probabilities (default: off)

-p --paramfile FILENAME

: read parameters from FILENAME (default: )

-a --alignment-order

: print sequences in alignment order rather than input order (default: off)

REFERENCE

Please cite Do, C.B., Mahabhashyam, M.S.P., Brudno, M., and Batzoglou, S. 2005. PROBCONS: Probabilistic Consistency-based Multiple Sequence Alignment. Genome Research 15: 330-340.

AUTHORS

Chuong Do <[email protected]>

: Wrote probcons in collaboration with Michael Brudno in the research group of Serafim Batzoglou, Department of Computer Science, Stanford University.

Charles Plessy <[email protected]>

: Wrote this manpage in DocBook XML for the Debian distribution.

COPYRIGHT

This program and its manpage are in the public domain.